胃黏膜树突状细胞与幽门螺杆菌相关性胃炎的关系

目的:研究胃黏膜树突状细胞在幽门螺杆菌(Hp)相关性胃炎发生中的作用。方法:内镜下收集正常/慢性胃炎胃黏膜标本,快速尿素酶试验、C14呼气试验和Warthin-Star-ry银染色检测Hp,Ficoll-Hypaque密度梯度离心法分离胃黏膜标本中的单个核细胞,进一步采用磁珠分选技术分离HLA-DR+DCs,分析DCs数量与Hp分布密度和胃黏膜炎症程度的关系。结果:Hp阴性正常胃黏膜中巨噬细胞平均含HLA-DR+DCs4.93%,Hp阳性正常胃黏膜中HLA-DR+DCs含量为17.93%,明显高于Hp阴性组(P<0.01);胃黏膜DCs数量与Hp分布密度成正相关(P<0.05);胃黏膜炎症程度为中度和重度的标本中HLA-DR+DCs含量明显高于轻度(P<0.05),中度和重度相比差异无统计学意义。结论:成功分离了胃黏膜DCs,并证实DCs与Hp相关性胃炎发生的早期阶段密切相关。

Relation between gastric dendritic cells and H pylori-associated gastritis

AIM: To investigate the effects of gastric dendritic cells(DCs) in H.pylori-associated gastritis.METHODS: Mononuclear cells were isolated from gastric samples of endoscoped healthy subjects or patients with gastritis by Ficoll-hypaquetechnique.HLA-DR+DCs were further isolated by MACS.Rapid urease test,warthin-starry dyer and С14-urea breath test were used to detect H.pylori.The relationship between the quantity of gastric DCs and H.pylori density or inflammatory background were analysed.RESULTS: The gastric mononuclear cells from H.pylori-infected normal gastric mucosa contained more HLA-DR+DCs(17.93 %) than that from noninfected subjects(4.93%).The quantity of gastric DCs is positively correlated with H.pylori density.The quantity of gastric DCs were significantly higher in moderate and high inflammatory gastric mucosa than that in low inflammatory gastric mucosa.There was no difference in the quantity of gastric DCs between moderate and high inflammatory gastric mucosa.CONCLUSION: Successfully isolated gastric DCs.DCs may play a role in the early stage of H.pylori-associated gastritis.