| 单次大剂量X线照射对大鼠胰腺的损伤作用 |
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| 引用本文： | 杨爱洁,何信佳,安永恒,苗亚丽. 单次大剂量X线照射对大鼠胰腺的损伤作用[J]. 齐鲁医学杂志, 2011, 26(4): 316-318 |
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| 作者姓名： | 杨爱洁 何信佳 安永恒 苗亚丽 |
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| 作者单位： | 1. 青岛大学医学院附属医院肿瘤科,山东青岛,266003
2. 济宁市第一人民医院 |
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| 基金项目： | 青岛市公共领域科技支撑计划项目 |
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| 摘 要： | 目的观察单次大剂量X线照射后,胰腺放射性损伤随照射时间的改变情况,为大分割放疗正常组织的保护提供实验依据。方法以6 MV-X线12 Gy照射大鼠腹部,在不同时间观察胰腺形态学改变,免疫组织化学方法测定Bcl-2、Bax蛋白的表达。结果照射后7 d腺细胞萎缩,个别坏死,14 d腺细胞间纤维增生,42 d腺细胞增生。照射后21 d胰岛细胞间出现纤维增生。随照射时间延长,腺细胞中Bcl-2蛋白表达阳性率升高,各实验组与对照组比较差异有显著性(t=7.44~148.40,P<0.001),随照射时间延长Bax蛋白表达由可疑阳性变为阴性,仅照射后2 d时与对照组相比差异有显著性(t=4.43,P<0.001)。随照射时间延长,胰岛细胞中Bcl-2蛋白表达阳性率升高,Bax表达阳性率降低,与对照组相比差异有显著性(t=7.11~85.80,P<0.001)。结论单次大剂量照射后,胰岛细胞间仅发生纤维增生;腺细胞初始有一过性急性损伤,然后出现代偿性修复,机制可能与随照射时间延长抗凋亡基因bcl-2表达渐增高,凋亡基因bax表达降低有关。
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| 关 键 词： | 辐射效应 放射损伤 胰腺 凋亡调节蛋白质 |
DAMAGE OF RAT PANCREAS AFTER A SINGLE LARGE-DOSE RADIATION |
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| YANG AI-JIE,HE XIN-JIA,AN YONGHENG,MIAO YA-LI. DAMAGE OF RAT PANCREAS AFTER A SINGLE LARGE-DOSE RADIATION[J]. Medical Journal of Qilu, 2011, 26(4): 316-318 |
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| Authors: | YANG AI-JIE HE XIN-JIA AN YONGHENG MIAO YA-LI |
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| Affiliation: | YANG AI-JIE,HE XIN-JIA,AN YONG-HENG,MIAO YA-LI (Department of Oncology,The Affiliated Hospital of Qingdao University Medical College,Qingdao 266003,China) |
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| Abstract: | Objective To observe the damage of pancreas after a single high-dose X-ray radiation,and provide experimental evidence for clinical application of hyper-fractionated radiotherapy. Methods The abdominal areas of rats were radiated by 12 Gy 6 MV X-ray.At different time points after radiation,the morphologic changes of pancreatic tissue were observed and the expressions of Bcl-2 and Bax proteins were measured with immunohistochemistry technique. Results Seven days after radiation exposure,acinar cells showed a... |
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| Keywords: | radiation effects radiation injures experimental pancreas apoptosis regulatory proteins |
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