| rSj26-Sj32融合蛋白用于慢性日本血吸虫病的诊断研究 |
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| 作者姓名： | Cai SF Li WG Wang M |
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| 作者单位： | 重庆医科大学附属第一医院传染病寄生虫病研究所,重庆,400016 |
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| 基金项目： | 重庆市科委地方病重大专项基金资助 |
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| 摘 要： | 目的:探讨rSj26-Sj32融合蛋白用于慢性日本血吸虫病的诊断价值.方法:用rSj26-Sj32融合蛋白和日本血吸虫成虫粗抗原(SjAWA)作为包被抗原建立酶联免疫吸附试验(ELISA)、斑点酶联免疫吸附试验(Dot-ELISA)和dipstick三种方法检测慢性日本血吸虫病患者血清特异性IgG抗体,同时以华支睾吸虫病、卫氏并殖吸虫病、泡型棘球蚴病和囊型棘球蚴病患者以及健康人血清作为对照.结果:rSj26-Sj32融合蛋白通过三种方法检测慢性日本血吸虫病的敏感性分别为95.00%、92.50%和92.50%;特异性分别为97.67%、95.35%和97.67%.结论:rSj26-Sj32融合蛋白可用于慢性日本血吸虫病的免疫诊断.
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| 关 键 词： | 日本血吸虫病 免疫诊断 融合蛋白 斑点酶联免疫吸附试验 dipstiek法 |
Study on diagnosis of chronic schistosomiasis japonicum with rSj26-Sj32 fusion protein |
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| Cai SF,Li WG,Wang M.Study on diagnosis of chronic schistosomiasis japonicum with rSj26-Sj32 fusion protein[J].Journal of Cellular and Molecular Immunology,2011,27(8):839-842. |
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| Authors: | Cai Shi-fei Li Wen-gui Wang Min |
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| Institution: | CAI Shi-fei,LI Wen-gui,WANG MinInstitute of Infectious and Parasitic Diseases,the First Affiliated Hospital of Chongqing Medical University,Chongqing 400016,China |
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| Abstract: | AIM: To study whether rSj26-Sj32 fusion protein can be used for diagnosis of chronic schistosomiasis japonicum.METHODS: ELISA,Dot-ELISA and dipstick were performed to detect the IgG in the sera of patients with chronic schistosomiasis japonicum using the antigens of rSj26-Sj32 fusion protein and SjAWA.The control sera were taken from health donors and the patients with clonorchiasis sinensis,paragonimiasis westermani,alveolar echinococcosis and cystic echinococcosis.RESULTS: The sensitivity of the three met... |
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| Keywords: | schistosomiasis japonicum immunodiagnosis fusion protein Dot-ELISA dipstick |
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