| 人乳癌细胞小球藻CVFad3基因的表达和作用 |
| |
| 引用本文： | 李艳君,薛美兰,郑征,赵敏,葛银林.人乳癌细胞小球藻CVFad3基因的表达和作用[J].齐鲁医学杂志,2011,26(4). |
| |
| 作者姓名： | 李艳君 薛美兰 郑征 赵敏 葛银林 |
| |
| 作者单位： | 1. 青岛大学医学院生物化学与分子生物学教研室,山东青岛,266021
2. 济源市第二人民医院检验科 |
| |
| 基金项目： | 教育部重点实验室开放基金 |
| |
| 摘 要： | 目的 构建真核表达载体pEGFP-C3-n-3,检测小球藻n-3脂肪酸脱氢酶基因CvFad3在人乳癌MCF-7细胞中的表达和作用.方法 通过RT-PCR法扩增得到CvFad3基因,将CvFad3基因cDNA插入到真核表达载体pEGFP-C3中,构建重组表达载体pEGFP-C3-n-3,并将其转染入MCF-7细胞中,设空载体pEGFP-C3为对照组.采用RT-PCR检测CvFad3基因的表达,MTT法分析CvFad3基因对MCF-7细胞增殖的影响,气相色谱检测CvFad3基因对MCF-7细胞n-3多不饱和脂肪酸含量的影响.结果 构建的重组载体pEGFP-C3-n-3转染入乳癌MCF-7细胞48 h后可检测到CvFad3基因mRNA的条带,而转染pEGFP-C3的MCF-7细胞48 h后检测不到CvFad3基因mRNA的条带.与对照组比较,转染pEGFP-C3-n-3的细胞MTT吸光度明显降低(t=6.039,P＜0.01);人乳癌细胞MCF-7细胞膜n-3多不饱和脂肪酸的含量显著升高.结论 重组载体pEGFP-C3-n-3构建成功,CvFad3基因能在MCF-7细胞内有效异源表达,且抑制了MCF-7细胞的增殖,增加了细胞膜n-3多不饱和脂肪酸的含量.
|
| 关 键 词： | 基因表达 普通小球藻 乳腺肿瘤 |
EXPRESSION AND EFFECT OF CHLORELLA VULGARIS CvFad3 GENE IN HUMAN BREAST CANCER CELLS |
| |
| LI YAN-JUN,XUEMEI-LAN,ZHENG ZHENG,ZHAO MIN,GEYIN-LIN.EXPRESSION AND EFFECT OF CHLORELLA VULGARIS CvFad3 GENE IN HUMAN BREAST CANCER CELLS[J].Medical Journal of Qilu,2011,26(4). |
| |
| Authors: | LI YAN-JUN XUEMEI-LAN ZHENG ZHENG ZHAO MIN GEYIN-LIN |
| |
| Institution: | LI YAN-JUN,XUE MEI-LAN,ZHENG ZHENG,ZHAO MIN,GE YIN-LIN (Department of Biochemistry and Molecular Biology,Qingdao University Medical College,Qingdao 266021,China) |
| |
| Abstract: | Objective To construct a eukaryotic expression vector pEGFP-C3-n-3,and study the expression and effect of n-3 fatty acid desaturase gene CvFad3 MCF-7 cells of human breast cancer. Methods n-3 fatty acid desaturase gene CvFad3 of Chlorella vulgaris was cloned by RT-PCR from Chlorella vulgaris.The cDNA of the gene was inserted into eukaryotic expression vector pEGFP-C3 to form a recombinant,it was then transfected into breast cancer cell MCF-7 by transfection reagent.The expression of n-3 gene was detected by... |
| |
| Keywords: | gene expression Chlorella vulgaris polyunsaturated fatty acids breast neoplasms |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
