环维黄杨星D对异丙肾上腺素致小鼠心肌肥厚保护作用的初步研究

目的：初步研究环维黄杨星D（Cvb—D）对小鼠心肌肥厚的保护作用。方法：皮下注射异丙肾上腺素（Iso）1mg·kg^-1，bid，连续14d，制备小鼠心肌肥厚模型，各治疗组分别给予环维黄杨星D（6，9，12mg·kg^-1）和阳性对照卡托普利（100mg·kg^-1）灌胃给药，末次给药24h后处死小鼠，测量小鼠心脏质量指数（全心质量／体质量）和左心室质量指数（左心室质量／体质量），并观察心肌组织病理形态学改变，以评价Cvb—D对小鼠心肌肥厚的保护作用。结果：与空白对照组比较，Iso模型组小鼠心脏重量指数明显升高，病理切片可见心肌肥厚改变。与Iso组相比，Cvb—D高剂量组能明显降低小鼠心脏质量指数和左心室质量指数（P〈0．05或0．01），病理切片可见损害较模型组减轻。结论：环维黄杨星D对Iso诱导的小鼠心肌肥厚具有一定的保护作用，其机制有待进一步研究阐明。

Primary study of Protective Effect of Cyclovirobuxinum D on Cardiac Hypertrophy Induced by Isoprenaline in Mice

To study the protective effect of cyclovirobuxinum D （Cvb-D） on cardiac hypertrophy induced by isoprenaline （Iso） in mice. Method： Mice cardiac hypertrophy model was established by administration of isoproterenol （Iso） 1 mg·kg^-1 sc, twice daily for 14 days. The therapy groups were intragastric administration of Cvb-D （6,9 and 12 mg·kg^-1） or captopril （100 mg· kg^-1 ）. The heart weight index （heart weight/body weight, HW/BW）, left ventricular weight index （left ventricular weight/body weight, LVW/BW） and myocardial morphological changes were determined to evaluate the protective effect of Cvb-D on cardiac hypertrophy. Result： Compared with those of control group, the HW/BW and LVW/BW of Iso group obviously increased, and the pathologic changes could be seen. Cvb-D （ 12 mg·kg^-1 ） could obviously decrease the heart indices in mice （P 〈0. 05 or P 〈0. O1 ） ,and also improve the pathologic damages of cardiac hypertrophy. Conclusion ： Cvb-D can inhibit cardiac hypertrophy induced by Iso, and the mech anism needs to be further studied.