血栓前体蛋白检测的临床意义

目的　探讨血浆中血栓前体蛋白 (TpP)浓度变化 ,在诊断DIC或前DIC状态中的意义。方法　应用酶联免疫技术测定 47例健康正常人 ,77例临床不同疾病血浆中TpP浓度 ,同时检测DIC相关指标 ,并进行统计学比较。结果　各临床病例DIC组、未发生DIC组血浆中TpP浓度 (11 2 0±5 10 )mg/L均显著较正常组高 (1 2 9± 1 0 2 )mg/L(P <0 0 0 1) ;DIC组 (13 2 0± 7 96)mg/L较未发生DIC组 (8 3 3± 6 3 0 )mg/L明显增高 (P <0 0 1) ,在DIC组TpP的异常率为 10 0 % ,比其他DIC相关指标异常率高。结论　据实验结果和相关文献表明血浆中TpP浓度测定在诊断DIC或前DIC状态中较之D二聚体、纤维蛋白原等方法具有更高的特异性和敏感性。同时在诊断DIC及其病程发展、疗效评估方面均有一定参考价值

Detection of thrombus precursor protein and its clinical significance

OBJECTIVE: To discuss the significance of the concentration change of plasma thrombus precursor protein (TpP) in the diagnosis of disseminated intravascular coagulation (DIC) or pre-DIC. METHODS: Enzyme linked immunosorbent assay (ELISA) was used in the detection of plasma TpP in patients with different diseases (77) and normal adults (47). At the same time, other correlated markers were detected and statistical comparison was proceeded. RESULTS: The concentration of TpP in the disease group (11.20 +/- 5.10) mg/L or the DIC (13.20 +/- 7.96) mg/L and non-DIC group (8.33 +/- 6.30) was obviously higher than that in the control group (1.29 +/- 1.02, P > 0.001). The concentration TpP in the DIC group was also obviously higher than that in the non-DIC group (P > 0.01). The rate of abnormal level of TpP in the DIC group was 100%, being higher than that of other markers related with DIC. CONCLUSION: Results of this study and pertinent literature showed that TpP detection has higher specificity and sensitivity than D-Dimer or fibrinogen (Fbg) assay in the diagnosis of DIC or pre-DIC. It provides more referable values for the diagnosis, treatment and prognosis of DIC.