尖锐湿疣患者皮损中HPV的检测分型及HPV6b L1变异分析

目的 探讨温州地区尖锐湿疣（CA）患者人乳头瘤病毒（HPV）感染型别及HPV6基因多态性。 方法 采用基因芯片检测73例CA患者皮损组织HPV 21种基因型的感染率，对所获得的11例患者的HPV6阳性者行HPV6b L1基因测序分析。 结果 73例CA患者标本中检出HPV阳性61例，总的阳性检出率为83.56％。在61例HPV阳性患者，其中单一型别阳性检出率为93.44％，单一型别感染中以HPV11（29.51％）型为主，其次为HPV6 （18.03％）型；混合型感染阳性检出率为6.56％，以HPV6+11型感染为主。此外，CA患者HPV 6b L1基因测序结果表明，6个位点的核苷酸存在多态性，其中810位点A/T变异存在于所有标本，可能为温州地方株突变。结论 HPV11、6型感染为CA的主要致病型别，HPV 6b L1基因存在多处突变。

Detection and typing of human papillomavirus (HPV) as well as mutation analysis of HPV 6b L1 gene in lesions from patients with condyloma acuminatum

Objective To investigate the genotype of HPV and L1 gene polymorphism of HPV subtype 6b in patients with condyloma acuminatum (CA) from Wenzhou region. Methods Gene chips were used to profile the genotype of HPV in tissue samples from 73 patients with CA. PCR was performed to amplify the L1 gene in 11 samples positive for HPV type 6 followed by sequencing. Results Of the 73 patients, 61 (83.56%) were positive for HPV DNA. Among the 61 HPV-positive patients, 93.44% were infected with single type of HPV, and 6.56% with mixed type of HPV. In patients infected with single type of HPV, type 11 predominated with a prevalence of 29.51% followed by HPV 6 (18.03%); in those infected with mixed type of HPV, 6and 11 were the most common genotypes. Besides, sequencing analysis revealed single nucleotide polymorphism at 6 sites of HPV 6b type L1 gene, and an A/T polymorphism at position -810 was present in all samples, which was considered to be a local mutation of HPV from Wenzhou region. Conclusions In Wenzhou region, HPV types 11 and 6 prevail in patients with CA, and multiple mutations are present in L1 gene of HPV 6b type.