| 成年大鼠脑室下层细胞表达GFR-α_1的免疫组织化学研究 |
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| 引用本文： | 刘方舟,杨宁,雷志年,曾水林,李涛,王磊. 成年大鼠脑室下层细胞表达GFR-α_1的免疫组织化学研究[J]. 神经解剖学杂志, 2005, 21(2): 199-202 |
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| 作者姓名： | 刘方舟 杨宁 雷志年 曾水林 李涛 王磊 |
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| 作者单位： | 东南大学,神经生物学研究所,南京,210009 |
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| 基金项目： | 江苏省自然科学基金(BK2002056)资助项目 |
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| 摘 要： | 观察胶质细胞源性神经营养因子受体-α1 (GFR -α1 )在成年大鼠脑室下层(SVZ)细胞的表达,探讨胶质细胞源性神经营养因子(GDNF)对SVZ细胞的作用。成年大鼠SVZ组织冰冻切片,采用GFR -α1 结合5- 溴脱氧尿苷(BrdU)的免疫组织化学单标记与双标记方法,对成年大鼠SVZ进行观察。在成年大鼠SVZ可见GFR- α1 阳性细胞、BrdU阳性细胞和GFR- α1 /BrdU双标记细胞,且三种阳性细胞的分布趋势相似。结果提示GDNF可能参与调节成年哺乳动物脑内SVZ细胞的增殖、分化和迁移。
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| 关 键 词： | GFR-α_1 免疫组织化学 脑室下层 成年大鼠 |
| 修稿时间： | 2004-10-08 |
IMMUNOHISTOCHEMICAL STUDY OF GFR-α1 EXPRESSING CELLS IN THE SUBVENTRICULAR ZONE OF ADULT RAT |
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| Liu Fangzhou,Yang Ning,Lei Zhinian,Zeng Shuilin,Li Tao,Wang Lei. IMMUNOHISTOCHEMICAL STUDY OF GFR-α1 EXPRESSING CELLS IN THE SUBVENTRICULAR ZONE OF ADULT RAT[J]. Chinese Journal of Neuroanatomy, 2005, 21(2): 199-202 |
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| Authors: | Liu Fangzhou Yang Ning Lei Zhinian Zeng Shuilin Li Tao Wang Lei |
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| Abstract: | In order to investigate the effect of glial cell line-derived neurotrophic factor (GDNF) in the subventricular zone (SVZ), the localization of GDNF family receptor-α_1 ( GFR-α_1 ) in cells of the SVZ of adult rat was examined with immunohistochemical staining for GFR-α_1 and/or 5-bromodeoxyuridine (BrdU). It was shown that GFR-α_1 and BrdU immunopositive cells and GFR-α_1/BrdU double-labeled cells were located in the SVZ. These results suggest that GDNF might have some important effects on the multiplication, differentiation and migration of cells in the SVZ of adult rat. |
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| Keywords: | GFR-α_1 immunohistochemistry subventricular zone adult rat |
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