条件培养基局部移植对大鼠脊髓损伤的修复作用

目的：观察骨髓间充质干细胞的条件培养基(BMSC-CM)局部移植对脊髓损伤修复的作用。方法采用改良的Allen 装置制作大鼠 T9脊髓损伤模型，随机分为对照组(脊髓损伤后局部微量注射生理盐水10μl)和实验组(脊髓损伤后局部注射 BMSC-CM 10μl)，两组均采用 BBB 评分、斜板实验法观察大鼠运动功能恢复情况，苏木精-伊红(HE)染色观察脊髓损伤处血管生长及空洞体积的改变情况。结果实验组大鼠的 BBB 后肢功能评分、斜板倾斜角度优于对照组(P <0．05)，脊髓损伤处空洞体积明显小于对照组，血管直径大于对照组。结论 BMSC-CM 能够减小脊髓损伤处的空洞体积和促进血管生长，从而促进脊髓损伤后神经功能的恢复。

Effects of conditioned medium local transplantation in repair of rat with spinal cord injury

Objective To investigate the promoting effects of bone marrow mesenchymal stem cells conditioned me-dium (BMSC-CM) local transplantation on function recovery of rat spinal cord injuries. Methods The spinal cord of adult rats was injured with modified Allen method, and the rats were randomly divided into 2 groups. The rats in the control group were subjected to the microinjection of saline solution (10 μl) into the spinal cord injuries site af-ter spinal cord injuries, and those in the experimental group were injected with the concentrated conditioned medi-um (10 μl) derived from the passage 3 BMSCs. The inclined plane test and Basso, Beattie and Bresnahan locomo-tor rating scale (BBB scale) were used to observe the recovery of rats’behavioral function after the transplantation. Hematoxylin-eosin (HE) staining to reflect the change of cavity volume and angiogenesis at the lesion epicenter. Results BMSC-CM treated animals showed better BBB scores and inclined plane scores than control animals, and this difference was statistically significant (P < 0. 05). BMSC-CM treated group showed reduced cavity volume and the blood vessels exhibited larger diameters compared to the control group. Conclusion BMSC-CM can promote the recovery of neurological function in spinal cord injury, reduce cavity volume, and improve blood vessel growth.