c-Met、VEGF在AFP阳性胃癌中的表达及意义

目的：探讨肝细胞生长因子受体c(c-Met)、血管内皮生长因子( VEGF)在甲胎蛋白( AFP)阳性胃癌中的表达,并探讨c-Met、VEGF与AFP阳性胃癌临床病理特征的关系。方法收集临床上AFP阳性胃癌组织标本共21例作为试验组,同时收集与该21例AFP阳性胃癌临床分期基本一致的普通胃癌组织标本作为对照组,通过免疫组织化学法检测其c-Met、VEGF的表达,分析比较两组肿瘤c-Met、VEGF表达的差别。结果试验组与对照组c-Met蛋白的阳性表达率分别为85．7%(18/21)、71．4%(15/21)；VEGF蛋白的阳性表达率分别为76．2%(16/21)、47．6%(10/21)。与对照组相比,试验组在VEGF、c-Met蛋白的表达上明显高于后者(P<0．05)。 Spearman等级相关性分析显示c-Met与VEGF在试验组中表达呈正相关( rs =0．621, P <0．05)。根据Lauren分型,发现在AFP阳性胃癌中肠型胃癌较其他类型易表达c-Met。未发现试验组中c-Met、VEGF表达与患者年龄、性别、肿瘤位置、分化程度、淋巴结转移、肝转移、生存时间、AFP水平等临床病理特征有相关性。结论试验组中c-Met及VEGF表达显著高于对照组,可能共同参与了AFP阳性胃癌的发生、发展过程,并可能具有协同促肿瘤血管生成作用。

The expression and significance of c-Met,VEGF in the AFP-positive gastric cancer

Objective To investigate the expression of c-Met and vascular endothelial growth factor( VEGF) in the alpha fetoprotein(AFP)-positive gastric cancer, and to explore the relationship between expression of c-Met and VEGF with clinicopathological features of AFP-positive gastric cancer. Methods A total of 21 cases AFP-positive gastric cancer tissues were selected as a test group, while collected 21 cases ordinary gastric,which had consistency with AFP-positive gastric cancer in stage,as a control group. The expressions of c-Met and VEGF were detected by immunohistochemistry , then the differences of c-Met, VEGF expression in two kinds of tumor were analyzed and compared. Results The positive expression rates of c-Met protein in AFP-positive gastric cancer and ordinary gas-tric carcinoma were 85. 7% (18/21) and 71. 4% (15/21),respectively. The positive expression rates of VEGF protein were 76. 2% (16/21) and 47. 6% (10/21), respectively. Compared with ordinary gastric cancer, AFP-positive gastric cancer was significantly higher than the former in the expression of VEGF and c-Met protein ( P<0. 05). Spearman rank correlation analysis showed that c-Met and VEGF expressions were positively correlated (rs=0. 621, P<0. 05) in the AFP-positive gastric carcinoma. AFP-positive gastric cancer, which was intestinal type by the Lauren classification, was more likely to express c-Met. The expressions of c-Met and VEGF were not sig-inificantly associated with the clinicopathological features in AFP-positive gastric cancer, such as age, sex, tumor location, degree of differentiation, lymph node metastasis, liver metastasis, survival time and AFP levels. Conclu-sion The expressions of c-Met and VEGF in AFP-positive gastric cancer tissues are significantly higher than ordi-nary gastric cancer tissues. They may be involved in the occurrence and development of AFP-positive gastric cancer and have synergistic effect on pro-angiogenesis.