IL-10R 基因多态性与乙型肝炎病毒感染慢性化的相关性

目的：探讨 IL-10R 基因多态性与乙型肝炎病毒(HBV)感染后慢性化的相关性。方法收集501例慢性HBV 感染患者和301例急性自限性 HBV 感染患者外周血标本,两组患者的年龄、性别和地区来源均相互匹配。 Snapshot方法检测 IL-10R 两个 tag SNP(rs2228055、rs2256111)的基因型；χ2检验用于比较两组间基因型及等位基因频率分布。结果 IL-10R 基因 rs2228055、rs2256111多态等位基因和基因型频率在两组中的分布均符合 Hardy-Weinberg 平衡。 IL-10R 基因 rs2228055位点 G/ G 基因型频率(7．2%)明显低于急性自限性 HBV 感染患者(13．0%),差异有统计学意义(P<0．05)；GG 基因型在 HBV 感染慢性化的风险是 AG + AA基因型的0.52倍(OR =0.52,95% CI：0．32~0．84)。结论IL-10R rs2228055位点 GG 基因型在 HBV 感染后的慢性化中发挥保护作用。

Association between IL-10R gene polymorphisms and spontaneous clearance of hepatitis B virus infection

Objective To examine whether IL-10R polymorphisms were associated with susceptibility to chronic hepatitis B virus (HBV)infection in a Chinese Han population. Methods A total of 501 chronic HBV-infected pa-tients as cases and 301 age-, sex-, and ethnically matched self-limiting HBV-infected individuals as controls were recruited in this study. Two tag SNPs (rs2228055 and rs2256111 of IL-10R) were genotyped in all subjects by u-sing Snapshot method. The frequencies of alleles and genotypes between the two groups were analyzed by χ2 analy-sis. Results All genotype and allele distributions in patients with C-HBV and patients with SL-HBV were in Har-dy-Weinberg equilibrium. The frequency of the rs2228055 GG genotype (7. 2% ) was significantly decreased in pa-tients with C-HBV as compared to SL-HBV patients (13. 0% ). In patients with chronic HBV infection, the risk of GG genotype in patients is 0. 52 times of that of the AG, AA genotypes patients ( OR = 0. 52,95% CI:0. 32 ~0. 84). Conclusion In this study, the results suggest that the rs2228055 GG genotype of IL-10R is associated with the susceptibility to chronic HBV infection in the Chinese Han population.