SIRT1在骨性关节炎滑膜中表达的相关研究

目的：探讨沉默信息调节因子2相关酶1(SIRT1)在骨性关节炎(OA)正常滑膜组织及细胞中表达的差异性。方法膝关节滑膜细胞培养传代,甲苯胺蓝染色观察细胞形态,Western blot 法检测滑膜组织及细胞中 SIRT1的表达情况；免疫组织化学法对滑膜细胞检测 SIRT1表达情况及表达部位。结果甲苯胺蓝实验显示 OA 及正常滑膜细胞无明显形态学差异；免疫细胞化学法显示 SIRT1在滑膜细胞胞质中广泛表达,染色强度 OA 组较正常组明显降低(t =20．208, P <0．01)；Western blot 法显示在滑膜细胞中 OA 组 SIRT1表达明显低于正常对照组((t =8．619,P <0．01)；在滑膜组织中 OA 组 SIRT1的表达亦明显低于正常对照组(t =7．664,P<0．01)。结论 SIRT1与 OA 导致滑膜炎的发生发展密切相关,为 SIRT1可以作为 OA 治疗的靶点提供一个新的理论依据。

The research of SIRT1 expression in osteoarthritis synovial membrane

Objective To investigate the expression of silent information regulator factor 2 related enzyme 1 (SIRT1) in osteoarthritis ( OA) synovial membrane tissue and cells. Methods Knee joint synovial membrane cells were cultured, and the synovial membrane cells morphology was detected by toluidine blue staining. The ex-pression of SIRT1 protein in osteoarthritis synovial membrane tissue and cells was detected by Western blot. The expression and distribution of SIRT1 protein was also detected by immunohistochemistry. Results Toluidine blue assay showed that OA and normal synovial cells had no obvious morphological difference; Immunohistochemistry showed that SIRT1 was widely expressed in synovial cells, the staining intensity of OA group was significantly de-creased compared with normal group (t = 20. 208, P < 0. 01); Western blot showed that the expression of SIRT1 in synovial cells in OA group was significantly lower than that in normal control group (t = 8. 619, P < 0. 01); The expression of SIRT1 in synovium of OA group was obviously lower than that in the normal group (t = 7. 664, P <0. 01). Conclusion The low expression of SIRT1 may play an essential role in OA progression, and the research provides a new theoretical basis for SIRT1 as therapeutic target in OA treatment.