内镜细胞刷几种低温灭菌方法的实验研究

目的：探讨几种低温灭菌方法对内镜细胞刷的灭菌效果。方法：内镜细胞刷浸染枯草杆菌黑色变种芽孢（ATCC9372），干燥后分别经戊二醛浸泡3h、4h、5h，戊二醛熏蒸3h、5h和环氧乙烷灭菌处理．采样培养7d。结果：内镜细胞刷经戊二醛浸泡3h、4h不能完全杀灭细胞刷污染的枯草杆菌黑色变种芽孢，浸泡5h，则可完全杀灭芽孢，达到灭菌合格；经戊二醛熏蒸3h、5h，不能完全杀灭细胞刷污染的枯草杆菌黑色变种芽孢；经环氧乙烷灭菌，能完全杀灭细胞刷污染的枯草杆菌黑色变种芽孢，达到灭菌合格。结论：戊二醛浸泡5h灭菌和环氧乙烷气体灭菌可有效杀灭内镜细胞刷污染的细菌芽孢，达到灭菌效果。

An Experimental Research of Low Temperature Sterilization Methods for Endoscopic Brushing Cells

Objective:To explore the efficient of low Temperature sterile methods for endoscopic brushing cells.Methods:The contaminated brushing cells with Bacillus var.niger spore were sterilised by six methods:A dipping in glutaraldehyde for 3h,B dipping in glutaraldehyde for 4h,C dipping in glutaraldehyde for 5h,D fumigated with glutaraldehyde for 3h,E fumigated with glutaraldehyde for 5h,F sterilized with ethylene oxide.The brushing cells were sampled and did germiculture for 7d.Results:The sterilization methods of A,B,D and E cannot killed Bacillus var.niger spore contaminated on brushing cells,but the metods of C and D can do.Conclusion: Two sterilization methods that dipping in dipping in glutaraldehyde for 5h and sterilized with ethylene oxide can kill all the spores of bacillus var.niger contaminated on endoscopic brushing cells.