龙葵抑制鸡胚绒毛尿囊膜血管新生的研究

目的:探讨龙葵对血管新生的影响。方法:应用鸡胚绒毛尿囊膜(CAM)血管新生模型进行研究,在鸡胚培养的第7天,分别给予高、中、低3个剂量(2·5,2·0,1·5mg·μL^-1)的龙葵水提物。培养至第9天收集数据进行评估。结果:龙葵给药组血管新生面积明显小于对照组(P<0·001)。病理形态学分析表明,给药组载体下CAM新生组织中新生血管数显著减少,并可见大血管结构破坏迹象。周围CAM组织中仅见少量新生血管。而对照组的载体下组织及周围CAM中均见大量新生血管。结论:龙葵可明显抑制鸡胚绒毛尿囊膜血管新生。

Experimental study of Solanum nigrumon inhibiting angiogenesis in chick chorioallantoic membrane

Objective: To study the inhibitory effect of Solanum nigrumon angiogenesis. Method: We examined the effects of S. nigrum on angiogenesis in the chick chorioallantoic membrane (CAM) model. On day 7 of chick embryo incubation, three concentrations of S. nigrum aqueous extracts were applied to CAMs, and their effects were evaluated on day 9. Result: The angiogenesis area was significantly smaller in the CAM treated with S. nigrum than that in the control group (P<0.001). Pathology analysis indicated that less angiogenesis occurred in the tissue of CAM under the filter paper treated with S. nigrum and the structure of large arteries was destroyed. The surrounding CAM showed a few angiogenesis formation. However, in the control group, a number of angiogenesis were observed. Conclusion: S. nigrum could inhibit the angiogenesis on CAM.