| IL-21对慢乙肝患者HBeAb生成与B淋巴细胞增殖的促进作用 |
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| 引用本文： | 胡春蓉,陈春波,谭晓菁,石统东. IL-21对慢乙肝患者HBeAb生成与B淋巴细胞增殖的促进作用[J]. 免疫学杂志, 2011, 0(2): 126-129,138 |
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| 作者姓名： | 胡春蓉 陈春波 谭晓菁 石统东 |
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| 作者单位： | 重庆市第九人民医院风湿免疫科;重庆医科大学病毒性肝炎研究所重庆医科大学附属第二医院感染病科; |
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| 基金项目： | 重庆市科委自然基金资助课题(CSTC,2010BB5381); 重庆市卫生局医学科研计划资助课题(2010-2-436) |
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| 摘 要： | 目的研究IL-21对慢性乙型病毒型肝炎患者外周血单个核细胞(PBMC)的促抗体分泌作用以及促B细胞增殖作用。方法以流式细胞术检测体外培养基线水平0 d和7 d的PBMC中B淋巴细胞的增殖及分化情况;用ELISA法检测培养上清液中HBeAb-IgG水平。结果①体外培养7 d后,各刺激组的B淋巴细胞均有明显增殖(P<0.01);与0 d时比较,IL-4与IL-21刺激组增殖最为显著(P<0.05)。②培养7 d后,各刺激组均可检测到HBeAb-IgG分泌,其中以IL-21刺激组产生的HBeAb水平明显增高,差异显著(P<0.05);而IL-4与IL-10刺激组无明显差别(P>0.05)。结论 IL-21具有促进B淋巴细胞增殖和HBeAb-IgG分泌的作用,在HBeAg(+)慢乙肝患者HBeAg血清学转换中可能发挥一定作用。
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| 关 键 词： | 慢性乙型病毒性肝炎 IL-21 IL-4 IL-10 B细胞 |
Effects of IL-21 on B lymphocytes proliferation and HBeAb production in chronic hepatitis B patients |
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| HU Chunrong,CHEN Chunbo,TAN Xiaojing,SHI TongdongRheumatic , Immunology Department,The Ninth People's Hospital of Chongqing,Chongqing ,China. Effects of IL-21 on B lymphocytes proliferation and HBeAb production in chronic hepatitis B patients[J]. Immunological Journal, 2011, 0(2): 126-129,138 |
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| Authors: | HU Chunrong CHEN Chunbo TAN Xiaojing SHI TongdongRheumatic Immunology Department The Ninth People's Hospital of Chongqing Chongqing China |
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| Affiliation: | HU Chunrong,CHEN Chunbo,TAN Xiaojing,SHI TongdongRheumatic and Immunology Department,The Ninth People's Hospital of Chongqing,Chongqing 400700,China |
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| Abstract: | To study the effects of IL-21 on B lymphocytes proliferation and HBeAb-IgG production in chronic hepatitis B patients,mononuclear cells were isolated from fresh peripheral blood of chronic hepatitis B patients by Ficoll-Hypaque density gradient centrifugation and stained with CFSE.The cells were then cultured in the presence of stimulus IL-4,IL-10 and IL-21 respectively for 1 week in vitro.Flow cytometry was used to detect the cell proliferation of B lymphocytes and ELISA was used to detect the HBeAb-IgG se... |
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| Keywords: | Chronic hepatitis B IL-21 IL-4 IL-10 B lymphocytes |
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