| Abstract: | Heterologous rabbit antifibroblast serum (AFS) against stromal fibroblasts of guinea pig bone marrow was obtained. Both in the cytological reaction for adherent cells and in the indirect immunofluorescence test AFS specifically bound fibroblasts and their precursor cells (but not macrophages) in monolayer primary cultures of bone marrow, thymus, and spleen cells, and also precursor cells of fibroblasts of the blood and peritoneal exudate of guinea pigs (in the cytotoxic reaction). Fibroblast precursor cells of the thymus were more sensitive to the action of AFS than splenic and bone marrow cells, which suggests a higher concentration of common tissue-specific antigens on the stromal mechanocytes of the thymus. Precursor cells of stromal fibroblasts in native cell suspensions were essentially more sensitive to the cytotoxic action of AFS than colony-forming fibroblasts from passage cultures.Laboratory of Immunomorphology, N. F. Gamaleya Institute of Epidemiology and Microbiology, Academy of Medical Sciences of the USSR, Moscow. (Presented by Academician of the Academy of Medical Sciences of the USSR P. A. Vershilova.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 85, No. 4, pp. 451–454, April, 1978. |
