胃黏膜下层胰岛移植物的生存率与功能的实验研究

目的 探讨胃黏膜下层胰岛移植的可行性.方法 应用雄性Wistar-Furth大鼠,STZ介导糖尿病大鼠模型.同种同基因大鼠体外胰岛分离纯化,分别移植进入糖尿病大鼠胃黏膜下层或肾被膜下,移植后不同时间段评估移植物生存率与代谢功能.结果 移植后早期移植胰岛被有效确认,并迅速分泌胰岛素逆转大鼠高血糖状况,糖耐受功能良好,与肾被膜下移植组比较,差异无显著意义.移植后4周,胃黏膜下移植组胰岛移植物胰岛素分泌功能逐渐丧失.结论 胃黏膜下胰岛移植技术具有良好的开发前景,如何提高其远期效果有待进一步考证.

Survival and metabolic function of gastric submucosally transplanted syngeneic rat islet graft

Objective To investigate the possibility that islets transplanted into the gastric submucosal layer of diabetic rat. Methods Male Wistar-Furth rats were used in the experiment. Diabetes was induced by the administration of streptozocin (STZ). Islets were isolated and purified in syngeneic rats. Islets mass was transplanted into the submucosal layer of the stomach. The graft survival and metabolic function were e-valuated in different phases. Results All STZ induced diabetic rats receiving 1500 islets in the submucosal layer of stomach returned to normoglycemia in 14 d after the transplantation. Histological examination of grafts showed numerous well-granulated insulin-containing cells in the submucosal layer. Compared with the renal subcapsular space transplantation group, no differences were found in the glucose tolerance changes. At the end of follow-up, hyperglycemia was observed in the rats of gastric submucosal transplant group. Conclu-sion The submucosal compartment of the upper GI tract is a viable site, which offers a safe and convenient place for transplantation islet in rodents.