Ploidy manipulation and induction of alternate cleavage patterns through inhibition of centrosome duplication in the early zebrafish embryo |
| |
| Authors: | Andrew O. Hasley Francisco Pelegri |
| |
| Affiliation: | Laboratory of Genetics, University of Wisconsin – Madison, Madison, Wisconsin |
| |
| Abstract: | Results : A heat pulse at a later time point during the first cell cycle (22 mpf, HS2) results in a high (>80%) frequency of embryos exhibiting a precise one‐cell division stall during the second cell cycle, inducing whole genome duplication. Coupled with haploid production, HS2 generates viable gynogenetic diploids with yields up to 4 times higher than those achieved through standard Heat Shock. The cell cycle delay also causes blastomere cleavage pattern variations, supporting a role for cytokinesis in spindle orientation during the following cell cycle. | |
| Keywords: | zebrafish heat shock centrioles whole genome duplication ploidy manipulation tetraploidy parthenogenesis gynogenesis |
|
|
