| 姜黄素对过氧化氢诱导的大鼠心肌细胞MCP-1表达的影响 |
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| 引用本文： | 郭守玉,龙明智.姜黄素对过氧化氢诱导的大鼠心肌细胞MCP-1表达的影响[J].南京医科大学学报,2010(4):442-444. |
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| 作者姓名： | 郭守玉 龙明智 |
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| 作者单位： | 南京医科大学第二附属医院心内科; |
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| 基金项目： | 江苏省六大高峰人才资助 |
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| 摘 要： | 目的:观察姜黄素(curcumine)对过氧化氢诱导的大鼠心肌细胞MCP-1表达的影响,探讨姜黄素在心肌细胞氧化应激中作用的可能机制。方法:以H2O2处理心肌细胞,构建心肌氧化损伤模型。并用姜黄素及H2O2共孵育心肌细胞,采用免疫印迹(Western blot)检测干预后不同时间MCP-1蛋白表达。结果:在干预后4、8、12、24、48h,H2O2组心肌细胞表达的MCP-1明显高于空白对照组(P<0.05),加入姜黄素共孵育后,各个时间点MCP-1的表达都明显下降(P<0.05)。结论:H2O2能诱导心肌细胞MCP-1蛋白表达,而姜黄素对H2O2诱导的心肌细胞MCP-1表达具有抑制作用,可能是其抗氧化应激的机制之一。
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| 关 键 词： | 姜黄素 心肌细胞 氧化损伤 单核细胞趋化蛋白-1 |
Effects of curcumine on expression of MCP-1 induced by H2O2 in myocardial cell |
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| GUO Shou-yu and LONG Ming-zhi.Effects of curcumine on expression of MCP-1 induced by H2O2 in myocardial cell[J].Acta Universitatis Medicinalis Nanjing,2010(4):442-444. |
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| Authors: | GUO Shou-yu and LONG Ming-zhi |
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| Institution: | Department of Cardiology/a>;the Second Affiliated Hospital of NJMU/a>;Nanjing 210011/a>;China |
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| Abstract: | Objective:To investigate the effects of curcumine on expression of monocyte chemoattractant protein-1 (MCP-1) induced by H2O2 in myocardial cell. To explore the possible mechanism of antioxygenation of curcumine in oxidative stress. Methods:In order to gain the oxidative injure cell model,cells myocardial with H2O2. Myocardial cells were incubated with curcumin and H2O2,then the expression of MCP-1 at different time points during the oxidative injure was detected by western blotting. Results:Western blottin... |
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| Keywords: | curcumine myocardial cell oxidative injure monocyte chemoattractant protein-1 |
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