重组人白介素-10对大鼠血管平滑肌细胞增殖和细胞周期的影响

目的　观察重组人白介素 10 (rhIL 10 )分别对肿瘤坏死因子 (TNF α)和血小板源性生长因子 (PDGF BB)刺激下离体大鼠胸主动脉血管平滑肌细胞增殖和细胞周期的影响。方法　体外培养大鼠胸主动脉血管平滑肌细胞 ,采用MTS/PES法确定血管平滑肌细胞的增殖状态 ,应用流式细胞术测定细胞周期。结果　与对照组相比 ,肿瘤坏死因子和血小板源性生长因子均对血管平滑肌细胞增殖具有明显的刺激作用 (P <0 0 5 )。rhIL 10单独应用对血管平滑肌细胞生长没有影响 (P >0 0 5 )。在TNF α和血小板源性生长因子分别刺激下 ,rhIL 10均可抑制血管平滑肌细胞的生长(P <0 0 5 )。流式细胞术测定的结果显示rhIL 10分别可以使TNF α和PDGF BB作用下的VSMCs大部分处于G0 /G1期 ,与对照组相比差异有显著性 (P <0 0 1)。结论　重组人白介素 10可抑制细胞因子和生长因子诱导的血管平滑肌细胞增殖

Effects of recombinant human recombinant interleukin-10 on proliferation and cell cycle of rat vascular smooth muscle cells

AIM To determine the effects of recombinant human interleukin 10 (rhIL 10) on rat vascular smooth muscle cells (VSMCs) proliferation by platelet derived growth factor and tumor necrosis factor α. METHODS Rat aortic VSMCs were cultured and treated with rhIL 10 or tumor necrosis factor α (TNF α) or platelet derived growth factor BB (PDGF BB) respectively. Proliferation was quantified by colormetric assay. Cell cycle analysis was performed by flow cytomertry. RESULTS Compared with control, TNF α and PDGF BB can stimulated VSMCs proliferation,respectively. rhIL 10 alone had no effect on VSMCs growth. With TNF α stimulation, rhIL 10, at dose as low as 10 μg·L -1 , inhibited VSMCs growth ( P< 0 05). With PDGF BB stimulation, rhIL 10, at dose as low as 10 ng·L -1 , inhibited VSMCs growth ( P< 0 05). Cell number in G 0/G 1 phase of PDGF BB and rhIL 10 co treatment group was higher than those of PDGF BB group ( P< 0 01) by flow cytometry analysis. The same results were observed in TNF α and rhIL 10 co treatment group ( P< 0 01). CONCLUSION The class Ⅱ cytokine receptor ligand, IL 10, inhibits TNF α and PDGF BB induced VSMCs proliferation, respectively. The class Ⅱ cytokine receptor may provide a novel therapeutic target in regulating vessel wall remodeling after vascular injury.