The inflammatory lesion of T cell line transferred experimental autoimmune encephalomyelitis of the Lewis rat: distinct nature of parenchymal and perivascular infiltrates |
| |
Authors: | Joseli Lannes-Vieira Jochen Gehrmann Georg W Kreutzberg Hartmut Wekerle |
| |
Institution: | (1) Abteilung für Neuroimmunologie, Max-Planck-Institut für Psychiatrie, Am Klopferspitz 18 a, D-82152 Martinsried, Germany;(2) Abteilung für Neuromorphologie, Max-Planck-Institut für Psychiatrie, Am Klopferspitz 18 a, D-82152 Martinsried, Germany;(3) Present address: Institute of Neuropathology, University Hospital, CH-8091 Zürich, Switzerland |
| |
Abstract: | We have investigated the T cell receptor (TCR) repertoire in the inflammatory infiltrates of T line-transferred experimental autoimmune encephalomyelitis (EAE) of the Lewis rats. Using a panel of TCR V -specific monoclonal antibodies (mAbs) and immunocytochemistry, we studied the nature of the T cells entering the central nervous system (CNS) after transfer of either myelin basic protein (MBP)-reactive, or MBP-reactive but non-encephalitogenic T cell lines. All the MBP-specific T cell lines predominantely used the V 8.2 TCR chain. T cell lines specific for the tuberculin purified protein derivative (PPD), using TCR V genes different from V 8.2, served as controls. We first studied the time course of T cells entering the CNS. In all recipient rats, small, but significant numbers of ![agr](/content/k6h1j8n76v0w1650/xxlarge945.gif) -TCR-expressing infiltrate cells appeared in the CNS within the first 24 h after T cell transfer. In animals injected with either type of MBP-reactive T cells, the early infiltrate cells were preferentially located within the parenchyma of the spinal cord, while in PPD T lineinjected rats, the lymphocytes were mostly found in the meninges. TCR V gene usage was examined on the peak of clinical disease. Six days after T cell transfer, the TCR repertoire used by infiltrating lymphocytes in general seemed to be highly diverse. None of the V isotypes examined (i.e. V 8.2, V 8.5 or V 10) was used by a major population of the ![agr](/content/k6h1j8n76v0w1650/xxlarge945.gif) -TCR-positive T cells. A more detailed, quantitative analysis of individual infiltrate compartments revealed, however, a preferential accumulation of V 8.2-positive T cells within the parenchyma. In contrast, perivascular infiltrating cells used V genes randomly. Our results confirm first that activated T lymphocytes enter the brain rapidly irrespective of their antigen specificity. Second, the data show that most of the perivascular infiltrate T cells in the acute EAE lesion are host-derived, recruited presumably from the recirculating T cell pool, while the encephalitogenic, V 8.2-positive T cells preferentially persist within the parenchyma.Abbreviations
EAE
experimental autoimmune encephalomyelitis
-
MBP
myelin basic protein
-
TCL
T cell line
Supported by the Brazilian Research Council (CNPq) |
| |
Keywords: | Central nervous system inflammation Brain immune surveillance T cell receptor usage |
本文献已被 SpringerLink 等数据库收录! |
|