血清瘦素、肿瘤坏死因子-α、IL-6在高脂诱导肥胖大鼠胰岛素抵抗中的作用

的：探讨高脂诱导肥胖模型大鼠血清瘦素、肿瘤坏死因子 α（TNF α）、白细胞介素6(IL 6)的水平及其在胰岛素抵抗中的作用。方法:大鼠高脂饮食8周后，以体重比对照组重30?g为标准，分出肥胖大鼠，继续饲养4周，空腹麻醉下，心脏取血，分离血清。葡萄糖氧化酶法测血清血糖；放射免疫法（RIA）测血清瘦素、胰岛素；酶联免疫法（ELISA）测血清TNF α、IL 6水平。以空腹胰岛素/血糖（Insulin/Glucose,I/G）比值作为胰岛素敏感指标。结果:与对照组相比，肥胖大鼠空腹胰岛素、I/G比值，瘦素、IL 6水平显著高于对照组(P均＜0.05)，血清TNF α水平无差别；肥胖大鼠I/G比值与血清瘦素、IL 6均密切相关(r=0.772,　P=0.036；　r=0.898,　P=0.015)，与TNF α不相关(r=0.341,　P=0.254)。结论:肥胖大鼠血清瘦素、IL 6显著增高，参与了肥胖相关的胰岛素抵抗，血清TNF α未增高，与肥胖相关的胰岛素抵抗无关。

Role of serum leptin, TNF-α and IL-6 on insulin resistance in high-fat diet induced obese rats

To evaluate the role of serum leptin, TNF α and IL 6 on insulin resistance in high fat diet induced obese rats. Methods: After fed with high fat diet for 8 weeks, the obese rats who were 30g heavier than controls were separated and fed for another 4 weeks. The blood samples were drawed by heart puncture. The serum levels of glucose were measured by glucose oxide peroxidase method, the levels of leptin and insulin by RIA, and the levels of TNF α, IL 6 by ELISA. The ratio of insulin versus glucose(I/G) was used as an indicator for insulin resistance. Results: Compared with the controls, the serum levels of leptin, IL 6 and I/G were higher in the obese rats, but the levels of TNF α were not different between two groups; The levels of leptin and IL 6,but not TNF α, were closely related to the parameters evaluating insulin resistance in obese rats. Conclusions: The serum levels of leptin and IL 6 are increased in high fat diet induced obese rats and contribute to insulin resistance. The levels of TNF α in obese rats are neither statistically increased nor related to insulin resistance.