中药狼疮方对狼疮样BXSB小鼠脾脏免疫系统和淋巴细胞亚群的影响

目的：研究中药狼疮方对狼疮样BXSB小鼠免疫系统和淋巴细胞亚群的影响，探讨狼疮方治疗SLE的免疫学机制。 方法： 采用BXSB小鼠模型，随机分为3组：狼疮方治疗组（每天0.5 mL狼疮方药液灌胃）、强的松治疗组（每天prednisone 0.173 mg/20 g BW溶于0.5 mL生理盐水灌胃），未治疗组（每天0.5 mL生理盐水灌胃），每组6只，疗程10周。另设与BXSB小鼠同基因的正常C57BL/6小鼠6只为正常对照组。分别采集上述各组小鼠外周血和脾组织进行检测。 结果： （1）未治疗组BXSB小鼠血清IgG和抗ds-DNA抗体水平及脾组织CD3、CD4、CD8、CD19、CD23阳性细胞百分比都显著高于正常对照组、狼疮方治疗组、强的松治疗组（P＜0.05或P＜0.01）。（2）经狼疮方或强的松治疗后，BXSB小鼠血清IgG、抗ds-DNA抗体水平及脾组织CD4、CD8、CD19、CD23阳性细胞百分比显著低于未治疗组（与未治疗组相比，P＜0.05或P＜0.01），且接近正常水平（与正常对照组比较，P＞0.05）。 结论： 狼疮样BXSB小鼠T、B细胞免疫功能上调。中药狼疮方可抑制狼疮样小鼠T、B淋巴细胞活化，减轻其高丙种球蛋白血症，减少体内自身抗体产生。

Effect of lupus recipe on immune system and lymphocyte subsets proliferation in splenic cells of lupus-prone BXSB mice

AIM: To investigate the effects of lupus recipe on immune system and lymphocyte subsets proliferation in splenic cells in BXSB mice. METHODS: Eighteen male BXSB mice model was used in the experiment. The model mice were divided into three groups: un-treated model group, lupus recipe (LR) treated group, and prednisone treated group. All model mice were killed in 10 weeks. The control group consisted of 6 syngeneic normal C57BL/6 male mice. The levels of total IgG and anti-dsDNA antibody in serum were detected by ELISA. The percentages of lymphocyte subsets (CD3 , CD4 , CD8 T lymphocytes and CD19 , CD23 B lymphocytes) were detected by using flow cytometry analysis. RESULTS: (1) The serum levels of total IgG and anti-dsDNA antibody in un-treated model group were higher than that in other groups. There was no differences among LR treated group, prednisone treated group and control group. (2) The percentages of CD3 , CD4 , CD8 T lymphocytes and CD19 , CD23 B lymphocytes in model group were obviously higher than that in normal control. (3) Compared to un-treated model group, the percentages of CD4 , CD8 T lymphocytes and CD19 , CD23 B lymphocytes in LR or prednisone treated group were significantly reduced, which closely reached the levels in normal group. CONCLUSIONS: The immune functions of T and B lymphocytes in BXSB mice are up-regulated. LR inhibits the activation of T and B lymphocytes, reduces the serum levels of IgG and auto-antibody production.