延长非协调性异种心脏移植存活时间的研究

目的 寻找延长非协调性异种心脏移植存活时间的方法。方法 实验分Ａ、Ｂ、Ｃ、Ｄ４组，将异种心脏（豚鼠－大鼠）移植于颈部。Ａ组：移植前受体用眼镜蛇蛇毒因子（ＣＶＦ）１５０ｕｇ．ｋｇ－１．ｄ＾－１，腹膜腔内注射（ｉ．ｐ），每天分２次，相隔３ｄ，共４次；Ｂ组：在Ａ组的基础上，移植前１ｈ加用己酮可可碱（ＰＴＸ）５０ｍｇ／ｋｇ（ｉ．ｐ），然后每隔６ｈ按２５ｍｇ／ｋｇ（ｉ．ｐ）至发生排斥反应为止；Ｃ组：在Ａ组的

Study of prolonging graft survival of discordant cardiac xenotransplantation

Objective To search the way of prolonging xenograft survival of discordant cardiac xenotransplantation.Methods All animals were divided into groups A,B,C and D receiving the following combined therapies respectively:cobra venom factor (CVF) in group A,CVF and pentoxifylline (FTX) in group B,CVF and CCS (CsA,CTX,MP)in group C,CVF,PTX and CCS in group D.The recipients were given CVF 150 μg/kg(i.P) twice daily 1,4 days before transplantation;PTX 50 mg/kg daily(i.P)1 h before transplamtation,then 25 mg/kg(i.P)in an interval of 6 h until rejection thereafter;CsA 10mg/kg(i.p) daily one day and 1 h before transplantation until rejection;CTX 40 mg/kg(i.p) one day before transplantation,then 10 mg/kg(i.P)daily 1 h before transplantation until rejection thereafter;MP 10 mg/kg(i.v)1 h before transplantation,then 1 mg/kg(i.m) daily until rejection thereafter.The expression of vascular cell adhesion molecular-1 (VCAM-1) of vascular endothelial cells (EC) in cardiac xenografts was detected by using immunohistochemical staining method.Results The mean survival time of cardiac xenografts was 57.5 h (group A),77.38 h(group B),79.13 h (group C) and 98.75 h(group D),respectively.Twenty-four h after transplantation.VCAM-1 expression in the groups B and D was not upregulated,but slightly up-regulated in group C and obvious in group A.At rejection,the up-regulation of VCAM-1 expression was mildest in group D and most obvious in group A.and VCAM-1 expression in group B was milder than that in group C (P＜0.05).Conclusions CVF combined with PTX and CCS could significantly prolong the survival time of cardiac xenograft.PTX mainly protected EC and CCS mainly inhibit inflammatory cell infiltration.Combined use of them could complement and reinforce mutually.