| 丹参酮ⅡA对乙醇诱导PC12细胞损伤的保护作用 |
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| 引用本文： | 邹晓静,孟宪芳,姚尚龙. 丹参酮ⅡA对乙醇诱导PC12细胞损伤的保护作用[J]. 中国药学杂志, 2006, 41(20): 1553-1556 |
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| 作者姓名： | 邹晓静 孟宪芳 姚尚龙 |
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| 作者单位： | 1. 华中科技大学同济医学院附属协和医院麻醉科,武汉,430022
2. 华中科技大学同济医学院神经生物学系,武汉,430030 |
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| 摘 要： | 目的探讨丹参酮ⅡA对PC12细胞乙醇毒性的保护作用。方法以PC12细胞为研究对象,分别用0.1,1,10μmol·L-1丹参酮ⅡA预处理细胞,以MTT检测细胞活性、乳酸脱氢酶(LDH)检测细胞损伤情况,以流式细胞学技术检测细胞凋亡及p53蛋白表达情况,观察丹参酮ⅡA对乙醇诱导的PC12细胞损伤的影响。结果乙醇显著降低PC12细胞活力(P<0.05),增加细胞LDH漏出(P<0.05),诱导细胞凋亡并上调促凋亡蛋白p53的表达(P<0.05);丹参酮ⅡA能显著减少乙醇对PC12细胞的毒性损伤(P<0.05),抑制细胞凋亡(P<0.05),降低p53蛋白的表达(P<0.05)。结论丹参酮ⅡA可能通过抑制促凋亡蛋白p53的表达,保护乙醇诱导的PC12细胞损伤。
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| 关 键 词： | 丹参酮ⅡA 乙醇 PC12细胞 凋亡 p53蛋白 |
| 文章编号： | 1001-2494（2006）20-1553-04 |
| 收稿时间： | 2005-09-06 |
| 修稿时间： | 2005-09-06 |
Protective Effects of Tanshinone Ⅱ A on Ethanol-induced Neurotoxicity in PC12 Cells |
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| ZOU Xiao-jing,MENG Xian-fang,YAO Shang-long. Protective Effects of Tanshinone Ⅱ A on Ethanol-induced Neurotoxicity in PC12 Cells[J]. Chinese Pharmaceutical Journal, 2006, 41(20): 1553-1556 |
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| Authors: | ZOU Xiao-jing MENG Xian-fang YAO Shang-long |
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| Affiliation: | 1.Department of Anesthesiology，Union Hospital of Tongji Medical College，Huazhong，University of Science and Technology，Wuhan 430022，China； 2.Department of Neurobiology，Tongji Medical College of Huazhong，University of Science and Technology，Wuhan 430030，China |
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| Abstract: | OBJECTIVE To investigate the protective effects of tanshinone ⅡA(Tan ⅡA)on ethanol-induced neurotoxicity in vitro.METHODS The toxicity model was established by treating PC12 cells with ethanol.PC12 cell viability was determined by MTT assay and leakage of lactate dehydrogenase(LDH).The cell apoptosis rate and the pro-apoptotic p53 protein expression were measured by flow cytometry.RESULTS Ethanol significantly impaired PC12 cell viability(P<0.05),increased LDH leakage(P<0.05),induced cell apoptosis and upregulated expression of p53(P<0.05).However,Tan ⅡA significantly reversed these changes(P<0.05).CONCLUSION Tan ⅡA might exert protection against ethanol-induced neurotoxicity through inhibiting the expression of p53 protein. |
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| Keywords: | tanshinone ⅡA ethanol PC12 apoptosis p53 protein |
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