| 胃癌高表达基因gcys-18的部分cDNA克隆 |
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| 引用本文： | 崔大祥,闫小君,苏成芝. 胃癌高表达基因gcys-18的部分cDNA克隆[J]. 医学争鸣, 1999, 20(9): 740-742 |
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| 作者姓名： | 崔大祥 闫小君 苏成芝 |
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| 作者单位： | 第四军医大学基因诊断技术研究所,陕西,西安,710033 |
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| 摘 要： | 目的：分离克隆测序胃癌高表达基因ｇｃｙｓ－１８的部分ｃＤＮＡ．方法：采用ｍＲＮＡ差异展示技术从胃癌ＧＣ７９０１与胃粘膜ＧＥＳ－１细胞株中分离出一差异表达基因片段，对其命名，并进行克隆、测序及ＧｅｎＢａｎｋ检索；以此片段作探针与细胞株总ＲＮＡ进行打点杂交．结果：从差示ＰＣＲ电泳凝胶中分离出大小为４１６ｂｐ的差异表达片段，命名为ｇｃｙｓ－１８；ＲＮＡ打点杂交证实ｇｃｙｓ－１８在ＧＣ７９０１中呈高表达，
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| 关 键 词： | 胃肿瘤 基因表达 克隆分子 序列分析 打点杂交 |
| 修稿时间： | 1998-10-31 |
Cloning part cDNA of gcys-18 overexpressed in gastric carcinoma |
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| CUI Da-Xiang,YAN Xiao-Jun,SU Cheng-Zhi. Cloning part cDNA of gcys-18 overexpressed in gastric carcinoma[J]. Negative, 1999, 20(9): 740-742 |
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| Authors: | CUI Da-Xiang YAN Xiao-Jun SU Cheng-Zhi |
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| Abstract: | AIM: To isolate, clone and sequence part cDNA of gcys 18 overexpressed in gastric carcinoma. METHODS: gcys 18, isolated from differential display gel between GC7901 and GES 1 by mRNA DD PCR, was cloned into T vector. As a probe, gcys 18 was hybridized to total RNAs of GC7901 and GES 1. It was then sequenced and searched against GeneBank. RESULTS: gcys 18 was isolated, cloned and found to be overexpressed in GC7901 and downexpressed in GES 1 by RNA dot blot. By sequencing, gcys 18 was confirmed to be 416 bp, partly similar to that of HEK5, and its accepted number in GeneBank is AF071057. CONCLUSION: Part cDNA of gcys 18 is isolated and cloned and it may be an important overexpressed gene in gastric carcinoma. |
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| Keywords: | stomach neoplasms gene expression cloning molecular sequence analysis RNA dot blot |
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