水杉总黄酮对大鼠脑缺血再灌注损伤的保护作用

目的 :观察水杉总黄酮对大鼠脑缺血再灌注损伤的保护作用。方法 :凝闭大鼠双侧椎动脉 4～ 5h后 ,夹闭颈总动脉 30min ,再灌注 90min ,造成大鼠脑缺血再灌注损伤模型 ,观察水杉总黄酮对脑组织中生化指标及脑电图的影响。结果 :缺血再灌注造成脑组织严重损伤 ,脑组织中水 ,Na⁺ ,Ca²⁺含量及丙二醛 (MDA)含量较对照组明显增高 ,而超氧化物歧化酶 (SOD)活力较对照组降低 ,并伴有脑电图异常和脑组织结构病理性改变。夹闭颈总动脉前 30min ip 25～ 100mg·kg^-1水杉总黄酮能降低脑组织中水 ,Na⁺ ,Ca²⁺ 含量及MDA含量 ,并提高SOD活力 ,对组织学检查及脑电图也有所改善。结论 :水杉总黄酮对脑缺血再灌注损伤具有保护作用。

Protective effects of total flavones of metasequosia on cerebral ischemia-reperfusion injury in rats

OBJECTIVE: To observe the protective effects of total flavones of metasequosia (TFM) on cerebral ischemia-reperfusion injury in rats. METHOD: The bilateral vertebral arteries of rats were occluded under anesthesia, and 4-5 h later the carotid arteries of rats in the conscious condition were occluded for 30 min followed by reperfusion for 90 min. The effects of TFM on the contents of water, Na+, Ca2+ in cortex, malondialdehyde (MDA) content and superoxide dismutase (SOD) activity in brain hemispheres, as well as the EEG activities were observed. RESULT: After 30 min ischemia and 90 min reperfusion, the contents of water, Na+ , Ca2+ and MDA were increased, and the SOD activity was reduced with abnormal EEG activity and ischemic injury in the brain tissues. TFM 25-100 mg x kg(-1) ip 30 min before the carotid arteries were occluded, decreased the elevated water, Na+, Ca2+ and MDA contents, increased the SOD activity, reduced the ischemic injury of brain tissue, and promoted the recovery of EEG activities. CONCLUSION: TFM has a protective effect on cerebral ischemia-reperfusion injury in rats.