| 辐射诱导溶瘤腺病毒联合放疗对宫颈癌细胞HeLa S3的作用效果 |
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| 引用本文： | 李笑梅,王海波,黄建. 辐射诱导溶瘤腺病毒联合放疗对宫颈癌细胞HeLa S3的作用效果[J]. 中国肿瘤生物治疗杂志, 2011, 18(6): 641-464 |
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| 作者姓名： | 李笑梅 王海波 黄建 |
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| 作者单位： | 1. 上海健康职业技术学院生物化学教研室,上海200237;上海交通大学医学院生物化学与分子生物学教研室,上海200025
2. 上海交通大学医学院生物化学与分子生物学教研室,上海,200025 |
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| 基金项目： | 国家自然科学基金资助项目（No.10979034，No.31071228）；上海市自然科学基金资助项目（No. 09ZR1416400） |
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| 摘 要： | 目的:构建受辐射诱导的EGR-1启动子调控的携带人TRAIL基因的新型溶瘤腺病毒Ad-EGR-TRAIL,研究其联合放疗对宫颈癌细胞株HeLa S3的杀伤效果.方法:构建重组腺病毒Ad-EGR-TRAIL,用腺病毒Ad-GFP检测对HeLa S3细胞的感染效率.CCK-8法检测Ad-EGR-TRAIL组、单纯放疗组以及...
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| 关 键 词： | TRAIL 腺病毒 基因疗法 放射治疗 凋亡 |
| 收稿时间： | 2011-08-15 |
| 修稿时间： | 2011-09-10 |
Effect of radiation-induced oncolytic adenovirus combined with chemotherapy on cervical cancer HeLa S3 cells |
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| LI Xiao-mei,WANG Hai-bo and HUANG Jian. Effect of radiation-induced oncolytic adenovirus combined with chemotherapy on cervical cancer HeLa S3 cells[J]. Chinses Journal of Cancer Biotherapy, 2011, 18(6): 641-464 |
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| Authors: | LI Xiao-mei WANG Hai-bo HUANG Jian |
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| Affiliation: | Department of Biochemistry, Shanghai Health Vocational and Technical College, Shanghai 200237, China; Department of Biochemistry and Molecular Biology, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China;Department of Biochemistry and Molecular Biology, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China;Department of Biochemistry and Molecular Biology, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China |
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| Abstract: | Objective : To construct a new radiation-induced, EGR-1 promotor-regulated, human TRAIL gene containing oncolytic adenovirus Ad-EGR-TRAIL, and to investigate the cytotoxicity effect of Ad-EGR-TRAIL combined with chemotherapy on cervical cancer HeLa S3 cells. Methods: Recombinant adenovirus Ad-EGR-TRAIL was constructed. HeLa S3 cells were infected with Ad-GFP, and infection efficiency was observed. The cytotoxicity effect of Ad-EGRTRAIL, radiotherapy (RAD), and Ad-EGR-TRAIL+RAD on HeLa S3 cells, as well as on normal human cervical cells, was examined by CCK-8 method. Results: Recombinant adenovirus Ad-EGR-TRAIL was successfully constructed. Ad-EGR-TRAIL showed the highest infection efficiency at MOI=100 in HeLa S3 cells. The inhibitory rates of HeLa S3 cells were (8.07±3.02)% and (23.02±4.03)% when Ad-EGR-TRAIL or RAD was used alone; however, the inhibitory rate reached (79.77±9.15)% when Ad-EGR-TRAIL and RAD were used in combination; and normal cervical cells did not significantly respond to the combination Ad-EGR-TRAIL and RAD therapy. Conclusion: Ad-EGR-TRAIL combined with chemotherapy can significantly kill cervical cancer HeLa S3 cells. |
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| Keywords: | TRAIL adenovirus gene therapy radiotherapy apoptosis |
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