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| 骨髓间充质干细胞体内外对胃癌细胞的趋向性 | |
| 引用本文： | 祝荫,程明,谢勇,陈江,罗时文,张焜和,吕农华. 骨髓间充质干细胞体内外对胃癌细胞的趋向性[J]. 中国肿瘤生物治疗杂志, 2011, 18(6): 647-652 |
| 作者姓名： | 祝荫 程明 谢勇 陈江 罗时文 张焜和 吕农华 |
| 作者单位： | 1. 南昌大学第一附属医院消化科,江西南昌,330006 2. 南昌大学第一附属医院骨科,江西南昌,330006 3. 南昌大学第一附属医院消化系疾病研究所,江西南昌,330006 4. 南昌大学第一附属医院消化科,江西南昌330006;南昌大学第一附属医院消化系疾病研究所,江西南昌330006 |
| 基金项目： | 国家自然科学基金资助项目（No. 81060198）；江西省教育厅项目（No. GJJ09429）；江西省科技厅项目（No. 2010BSA12800） |
| 摘 要： | 目的:探讨人骨髓间充质干细胞(human bone marrow-derived mesenchymal stem cell,hBMSC)对胃癌细胞的趋化性,为将MSC研发成为胃癌基因治疗载体提供实验依据.方法:骨髓培养法分离培养hBMSC并进行流式细胞术鉴定.Transwell实验检测hBMSC对胃癌MKN45细胞和人成纤维细胞(human fibroblast,hFB)的趋化能力.建立MKN45细胞裸鼠移植瘤模型,感染Lenti-EGFP(MOI为50)的hBMSC或hFB细胞经尾静脉注射入荷瘤小鼠,荧光显微镜下观察移植瘤组织及各脏器GFP的表达.结果:培养第3代的hBMSC细胞CD44、CD105阳性率为(96.7±1.84)％、(98.1±0.95)％,而CD34、CD45表达阴性.hBMSC细胞向MKN45细胞的趋化能力明显强于胃上皮细胞GES-1组及空白对照细胞[(239.5-54.3)vs(43.57±4.6)、(37.3±4.7)个,P＜0.01],且hBMSC向MKN45细胞的趋化能力明显强于hFB细胞向MKN45细胞的趋化能力[(239.5 ±54.3)vs(27.7 ±16.7),P＜0.01].与hFB相比,hBMSC对胃癌移植瘤组织具有明显趋向性；hBMSC组移植瘤组织内可见GFP表达,移植瘤小鼠部分肝脏(20％)及肺脏(20％)有GFP表达,但较移植瘤组织内GFP表达率和强度均低(P＜0.05).结论:hBMSC在体内外对胃癌细胞均有特异性趋化作用,有望研发为胃癌基因治疗的良好载体. |
| 关 键 词： | 胃癌 间充质干细胞 细胞载体 趋化作用 |
| 收稿时间： | 2011-08-20 |
| 修稿时间： | 2011-09-26 |
Chemotaxis of bone marrow-derived mesenchymal stem cells to gastric cancer cells in vivo and in vitro |
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| ZHU Yin,CHENG Ming,XIE Yong,CHEN Jiang,LUO Shi-wen,ZHANG Kun-he and LV Nong-hua. Chemotaxis of bone marrow-derived mesenchymal stem cells to gastric cancer cells in vivo and in vitro[J]. Chinses Journal of Cancer Biotherapy, 2011, 18(6): 647-652 | |
| Authors: | ZHU Yin CHENG Ming XIE Yong CHEN Jiang LUO Shi-wen ZHANG Kun-he LV Nong-hua |
| Affiliation: | Department of Gastroenterology, First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi, China;Department of Osteology, First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi, China;Institute of Digestive Diseases, First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi, China;Institute of Digestive Diseases, First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi, China;Institute of Digestive Diseases, First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi, China;Institute of Digestive Diseases, First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi, China;Department of Gastroenterology, First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi, China;Institute of Digestive Diseases, First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi, China |
| Abstract: | |
| Keywords: | gastric cancer mesenchymal stem cell cell vehicle chemotaxis |
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