| 双Tac启动子的构建及其对人白细胞介素2在大肠杆菌中表达的影响 |
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| 引用本文： | 张大军,冯博,皇甫永穆. 双Tac启动子的构建及其对人白细胞介素2在大肠杆菌中表达的影响[J]. 华中科技大学学报(医学版), 1993, 0(4) |
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| 作者姓名： | 张大军 冯博 皇甫永穆 |
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| 作者单位： | 同济医科大学实验医学研究中心医学分子生物学研究室，同济医科大学实验医学研究中心医学分子生物学研究室，同济医科大学实验医学研究中心医学分子生物学研究室 武汉 430030，武汉 430030，武汉 430030 |
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| 摘 要： | 采用基因工程技术,在表达质粒 PTLIL-2中于人白细胞介素 2(interleukin 2,IL-2)cD-NA 片段前插入了来自质粒PDR540的含Tac 启动子的片段,并适当调整SD 序列与ATG 密码之间的距离,构建成含双Tac 启动子和双SD 序列的质粒 PTLIL-2DT。结果该重组质粒的IL-2 基因在大肠杆菌中表达效率提高了4倍。本文对重组 IL-2抽提和生物活性恢复的处理方法也作了探讨。
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| 关 键 词： | 操纵子 白细胞介素2 基因表达 大肠杆菌 |
Construction of Double Tac Promoters and Their Effect on the Expression of Human IL-2 cDNA in E.coli |
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| Zhang Dajun,Huangfu Yongmu,Feng Bo. Construction of Double Tac Promoters and Their Effect on the Expression of Human IL-2 cDNA in E.coli[J]. Journal of Huazhong University of Science and Technology(Health Sciences), 1993, 0(4) |
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| Authors: | Zhang Dajun Huangfu Yongmu Feng Bo |
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| Affiliation: | Zhang Dajun,Huangfu Yongmu,Feng BoDepartment of Medical Molecular Biology,Research Centre of Experimental Medicine,Tongji Medical University,Wuhan 430030 |
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| Abstract: | We inserted Tac promoter sequence derived from plasmid PDR540 into the upstream of IL-2 cDNA in PTLIL-2 and reduced the distance between SD sequences and initiation ATG to construct a plasmid PTLIL-2DT which contained double Tac promoters and double SD sequences. It was found that the synthesis of IL-2 in PTLIL-2DT was 4 times more efficient than that in PTLIL-2. Besides, techniques for the extraction and renaturation of recombinant IL-2 from bacteria cells have also been discussed. |
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| Keywords: | interleukin-2 genetic engineering high-level expression renaturation |
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