FVB小鼠精子冷冻及体外受精的研究

目的：探索FVB小鼠及FVB遗传工程小鼠种系保存的新途径.方法：用孕马血清和人绒毛膜促性腺激素（HCG）超排FVB小鼠,并分别用复苏精子和新鲜精子进行体外受精并将受精卵移植到假孕雌鼠输卵管中,比较两种精子的受精率;对注射HCG后13,15,17h不同时间采集FVB小鼠的卵母细胞进行体外受精,比较其受精率及体外培养至2细胞情况.结果：新鲜精子的受精率（62.4%）略高于冷冻复苏精子的受精率（58.1%）;在注射HCG 13～15 h后取FVB小鼠卵母细胞其受精率效果较好,受精率（60.2%、61.6%）显著高于17h取卵的受精率（51%）,但13,15,17 h采集卵母细胞体外受精后胚胎发育至2细胞及异常卵数无显著性差异.结论：本研究将为FVB小鼠及FVB遗传工程小鼠的种系保存、繁殖提供了基础.

Study on sperm cryopreservation and in vitro fertilization in FVB mice

Objective: To explore a new approach to preserving FVB mice strains and FVB genetic engineering mice strains. Methods:FVB mice were superovulated with PMSG and HCG. The ova were fertilized with fresh and post-thaw sperms respectively, and the zygotes were transplanted into the oviducts of the pseudopregnant mice. The fertilization rate and pregnancy rate of both fresh and post-thaw sperms were compared. The pregnant rate and the number of 2-cell embryo were determined in FVB mice 13,15, and 17 hours after the injection of HCG. Results:The fertilization rate of fresh sperm was higher than that of post-thaw sperm (62.4% vs. 58.1%),but the difference was not significant. The fertilization rate 13 and 15 hours after the injection of HCG was significantly higher than that 17 hours after the injection,respectively (60.2% vs. 51%, 61.6% vs. 51%).There was no significant difference in the number of 2-cell embryo among FVB mice 13,15,and 17 hours after fertilization. Conclusion:This study provides the foundation for preserving FVB mice strain and FVB genetic engineering micestrain and breeding them.