A型肉毒毒素对痉挛性瘫痪大鼠腓肠肌作用的实验研究

目的:研究A型肉毒毒素注射致痉挛瘫痪大鼠腓肠肌及相关结构超微结构的改变.方法:150只大鼠随机分A、B、C、D4组:A组为30只,单纯颅骨钻孔:B组为40只、C组为50只、D组为30只,各组均以适宜电流刺激破坏左侧锥体束制备痉挛瘫痪模型.C组、D组给予腓肠肌痉挛肌肉注射A型肉毒毒索6u/kg/肌群;D组,于注射后辅以运动训练.B组为对照组,给予注射等体积生理盐水.各组于注射后不同时间进行神经行为学检测、肌肉及相关组织结构透射电镜检查.结果:C组和D组的神经、肌肉组织改变情况均较对照B组明显.C组和D组,均观察到神经芽生现象.训练后D组大鼠神经行为学及超微结构改善明显.肌膜两侧呈现不同的病理改变.结论:A型肉毒毒素肌肉注射导致的超微结构改变较单纯肌痉挛造成的改变更为明显;肌细胞膜对肉毒毒素具有阻隔作用,A型肉毒毒素可能有诱发神经芽生作用.运动训练对大鼠神经行为学有明显改善,运动训练可促进病变肌肉超微结构改善.

The experimental study on effects of BTX-A on gastrocnemius muscle of spastic paralytic rat's

Objective: To study the effects of botulinum toxin-A (BTX-A)on the ultrastructural change of gastrocnemius muscle and relative tissue of spastic paralytic rats. Method: A total of 150 rats were randomly divided into four(A,B,C,D) groups. All rats of 4 groups were drilled a holl on the skull; rats of B,C,D groups were underwent electrical destroy at left pyramidal tract; after that saline and BTX-A were injected into gastrocnemius muscle of spastic paralytic group B rats and group C and D rats respectively; then let the group D rats to exercise in rolling cage. The ultrastructural changes of gastrocnemius muscle and related tissue of spastic paralytic rats in different period were studied by transmission electronic microscopy. Result: The ultrastructural changes of muscle and related tissues were more obviously in group C and D than that in group B. After 3 months the behaviors and ultrastructures of muscles and related tissues were improved in group D. Nerve sproutings were also observed in group C and D under transmission electronic microscopy. The pathologic changes were different at both sides of muscle membranes. Conclusion: The changes of ultrastructure in muscles after injection of BTX-A are more distinct than that in rats of single spasmodic models. The membranes of muscle cells can seemly prevent the penetrating of BTX-A. BTX-A maybe induces the nerve sprouting. Exercises can obviously improve the function of nerve and movement, and can promote the resume of muscular ultrastructure.