Hp感染与胃癌中癌基因和抑癌基因蛋白表达的关系 |
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引用本文: | 祖毅,蜀津,钟正福,代红莹,唐治贵,王新.Hp感染与胃癌中癌基因和抑癌基因蛋白表达的关系[J].中国肿瘤临床与康复,2001,8(2):9-11. |
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作者姓名: | 祖毅 蜀津 钟正福 代红莹 唐治贵 王新 |
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作者单位: | 1. 重庆市第八人民医院 2. 重庆市第二人民医院 |
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基金项目: | 重庆市卫生局科研基金资助课题(96015) |
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摘 要: | 目的研究Hp感染与胃癌发生的关系及其可能的致癌机制。方法经内镜和病理诊断的胃癌91例,用快速尿素酶试验,改良W-S银染色和PCR方法检测上述标本中Hp;以S-P免疫组织化学法检测P21、P53、C-mcy及C-erbB-2,并用阳性表达积分半定量和免疫组化图像分析进行比较。结果Hp阳性胃癌组(44例)的P21、P53、C-myc及C-erbB-2表达阳性率较Hp阴性胃癌组(47例)为高。Hp阳性组这4种癌基因蛋白的阳性表达积分、平均光密度和积分光密度均显著高于Hp阴性组(P<0.05~0.01)。结论Hp感染与胃癌中P21、P53、C-myc及C-erbB-2的异常表达密切相关,提示Hp感染可使胃粘膜细胞的癌基因活化和抑癌基因突变或失活,促进胃粘膜细胞恶性转化而导致胃癌发生。
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关 键 词: | 幽门螺杆菌感染 胃肿瘤 癌基因蛋白 免疫组织化学 |
文章编号: | 1005-8664(2001)02-09-03 |
修稿时间: | 2000年3月22日 |
Relationship between
Helicobacter pylori infection and expressions of onceogene and tumor suppressor gene
protein in gastric cancer |
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Abstract: | Objective To investigate the possible role of Hel icobacter pylori(HP) infection in the development of gastric cancer with overexp ression of P21,P53,C-myc and C-erbB-2.Methods A total of 91 patients had gastric cancer,who were diagnosed with endoscopy and histopathology .HP were determined by rapid urease test,Warthin-Starry silver stain and polyme rase chain reaction,and expression of P21,P53,C-myc and C-erbB-2 were detecte d by immunohistochemicology (Streptaovidin/Peroxidase,SP) method,and were evalua ted with positive expressive integrality (PEI),the mean optical density (MOD) an d the integral optical density (IOD) in computer assisted image analysis system. Results The positive expressive rates of P21,P53,C-myc and C- erbB-2 were higher in HP positive cases than those in HP negative cases.The PEI ,MOD and IOD of P21,P53,C-myc and C-erbB-2 were significantly higher in HP po sitive cases than those in HP negative cases(P<0.05~0.01).Conclusions HP infection activates oncogene and inactivates tumor suppresser gene an d increase overexpression of P21,P53,C-myc and C-erbB-2,which may be involved in development of gastric cancer. |
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Keywords: | Helicobacter pylori infection stomach neoplasms oncogene protein immunohistochemicology |
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