Hp感染与胃癌中癌基因和抑癌基因蛋白表达的关系

目的研究Hp感染与胃癌发生的关系及其可能的致癌机制。方法经内镜和病理诊断的胃癌91例，用快速尿素酶试验，改良W-S银染色和PCR方法检测上述标本中Hp；以S-P免疫组织化学法检测P21、P53、C-mcy及C-erbB-2，并用阳性表达积分半定量和免疫组化图像分析进行比较。结果Hp阳性胃癌组(44例)的P21、P53、C-myc及C-erbB-2表达阳性率较Hp阴性胃癌组(47例)为高。Hp阳性组这4种癌基因蛋白的阳性表达积分、平均光密度和积分光密度均显著高于Hp阴性组(P<0.05～0.01)。结论Hp感染与胃癌中P21、P53、C-myc及C-erbB-2的异常表达密切相关，提示Hp感染可使胃粘膜细胞的癌基因活化和抑癌基因突变或失活，促进胃粘膜细胞恶性转化而导致胃癌发生。

Relationship between Helicobacter pylori infection and expressions of onceogene and tumor suppressor gene protein in gastric cancer

Objective　To investigate the possible role of Hel icobacter pylori(HP) infection in the development of gastric cancer with overexp ression of P21,P53,C-myc and C-erbB-2.Methods　A total of 91 patients had gastric cancer,who were diagnosed with endoscopy and histopathology .HP were determined by rapid urease test,Warthin-Starry silver stain and polyme rase chain reaction,and expression of P21,P53,C-myc and C-erbB-2 were detecte d by immunohistochemicology (Streptaovidin/Peroxidase,SP) method,and were evalua ted with positive expressive integrality (PEI),the mean optical density (MOD) an d the integral optical density (IOD) in computer assisted image analysis system. Results　The positive expressive rates of P21,P53,C-myc and C- erbB-2 were higher in HP positive cases than those in HP negative cases.The PEI ,MOD and IOD of P21,P53,C-myc and C-erbB-2 were significantly higher in HP po sitive cases than those in HP negative cases(P<0.05～0.01).Conclusions　HP infection activates oncogene and inactivates tumor suppresser gene an d increase overexpression of P21,P53,C-myc and C-erbB-2,which may be involved in development of gastric cancer.