Chromosomal polymorphism of the yeast Yarrowia lipolytica and related species: electrophoretic karyotyping and hybridization with cloned genes |
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Authors: | Elena Naumova Gennadi Naumov Philippe Fournier Huu-Vang Nguyen Claude Gaillardin |
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Institution: | (1) State Institute for Genetics and Selection of Industrial Microorganisms, 1 Dorozhnyi 1, 113545 Moscow, Russia;(2) Collection de Levures d'Intérêt Biotechnologique INRA-INA-CNRS, Centre de Biotechnologies Agro-Industrielles, Institut National Agronomique, F-78850 Thiverval-Grignon, France |
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Abstract: | Significant differences in electrophoretic karyotyping patterns were found among 27 strains of Y. lipolytica. Twenty-one of these strains were classified into four groups of similar karyotypes while six strains showed unique karyotypes. Chromosomal DNAs of different strains were hybridized with cloned genes of Y. lipolytica (URA3, LEU2, ARS18 and ARS68), which revealed four different bands in most strains. We conclude that the haploid chromosome number of Y. lipolytica is at least four, and possibly five or six. Electrophoretic karyotyping and hybridization with cloned genes of Y. lipolytica provided evidence of a large divergence between Y. lipolytica and related species of Saccharomycopsis, Endomycopsella and Endomyces. |
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Keywords: | Karyotype Yarrowia lipolytica Chromosome polymorphism Hybridization |
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