| 谷氨酸诱导转Bax基因PC12细胞凋亡及神经生长因子的保护作用 |
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| 引用本文： | 康静琼,阮旭中,王伟,张苏明. 谷氨酸诱导转Bax基因PC12细胞凋亡及神经生长因子的保护作用[J]. 中风与神经疾病杂志, 2001, 18(2): 75-77 |
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| 作者姓名： | 康静琼 阮旭中 王伟 张苏明 |
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| 作者单位： | 同济医科大学附属同济医院神经科, |
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| 基金项目： | 国家自然科学基金资助项目 (No 39700047) |
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| 摘 要： | 目的探讨谷氨酸诱导转Bax基因PC12细胞凋亡及神经生长因子（NerveGrowthFactor,NGF）的保护作用。方法应用脂质体介导细胞转染方法进行Bax基因转染PC12细胞，结合流式细胞术和TUNEL法检测了谷氨酸处理前后转Bax基因PC12细胞的凋亡及NGF的保护作用。结果与对照组相比，谷氨酸处理转Bax基因PC12细胞凋亡峰增高，TUNEL阳性标记细胞增多；用NGF处理后该组细胞凋亡峰减低，TUNEL阳性标记细胞数减少。差异均有显著意义。结论谷氨酸处理转染Bax基因PC12细胞凋亡增加，NGF可以保护谷氨酸诱导的转染Bax基因PC12细胞凋亡。
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| 关 键 词： | 谷氨酸； Bax； 凋亡； NGF |
| 文章编号： | 1003-2754(2001)02-0075-03 |
| 修稿时间： | 2000-10-11 |
A study on glutamate-induced apoptosis of transfecting Bax PC12 cell line and neuroprotection of nerve growth factor |
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| KANG Jing qiong,RUAN Xu zhong,WANG Wei,at al.. A study on glutamate-induced apoptosis of transfecting Bax PC12 cell line and neuroprotection of nerve growth factor[J]. Journal of Apoplexy and Nervous Diseases, 2001, 18(2): 75-77 |
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| Authors: | KANG Jing qiong RUAN Xu zhong WANG Wei at al. |
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| Abstract: | Objective To study the glutamate-induced apoptosis of transfecting Bax PC12 cell line and neuroprotective effect of nerve growth factor. Methods Using lipofectin-mediated gene transfer method to construct bax transfected PC12 cell line and to detect apoptosis of subject groups by flowcytometry ananysis(FCA) and terminal deoxynucleotidyl transferase mediated dUTP-biotin nick end labeling(TUNEL). Results The glutamate-induced apoptotic peak of transfecting Bax PC12 cell was higher than that of the same group treated with NGF. The differences were statistically significant(P<0.01). Conclusions The results suggest that glutamate can increase apoptosis of transfecting Bax PC12 cell and NGF has effect of neuroprotection on this transfected cell line. |
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| Keywords: | Glutamate Bax Apoptosis NGF |
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