氯化镧诱导脑胶质瘤SHG44细胞凋亡作用的机制

目的探讨氯化镧对人脑胶质瘤细胞株SHG44细胞诱导凋亡及其作用机制。方法体外培养人胶质瘤细胞，流式细胞仪、电镜检查细胞周期和超微结构的改变，原位杂交技术及免疫组化染色技术检测bcl-2、bax基因、蛋白表达情况。结果氯化镧能促进入脑胶质瘤SHG44细胞发生凋亡，明显增加G1期DNA含量，减少S期DNA含量。使bax基因、蛋白表达显著增强，bcl-2、bax基因蛋白表达显著减弱（P〈0．05）。结论氯化镧可抑制人脑胶质瘤SHG44细胞的生长，并促进其凋亡。

Experiment research of apoptotic effects on human brain glioma SHG44 with LaCl3

Objective: To investigate the feasibility and mechanism of LaCl_3 induced apoptosis in human glioma SHG44.Methods: The human glioma SHG44 cells were cultured in vitro,the cell circle and ultrastructure were detected by flow cytometry and electron microscope.The protein expression and translation of bax,bcl-2 gene were detected by immunohistochemistry and in situ hybridization methods.Results: The LaCl_3 could inhibit the growth of SHG44 and induce apoptosis.The DNA content of SHG44 increased in G_1 cycle and decreased in S cycle obviously.Typical apoptosis cells appeared.Both of protein expression and mRNA translation of bcl-2 gene decreased,the bax gene protein expression and mRNA translation increased(P< 0.05).Conclusion: LaCl_3 can inhibit the proliferation of SHG44 cells in vitro and cause apoptosis.