| 体外AGEs诱导培养的视网膜Müller细胞增殖活性和分泌VEGF的改变 |
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| 引用本文： | 叶辉,徐格致,王文吉.体外AGEs诱导培养的视网膜Müller细胞增殖活性和分泌VEGF的改变[J].国际眼科杂志,2008,8(11):2217-2218. |
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| 作者姓名： | 叶辉 徐格致 王文吉 |
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| 作者单位： | 江苏省人民医院眼科;复旦大学附属眼耳鼻喉科医院 |
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| 摘 要： | 目的:探讨体外Mller细胞在糖基化终末产物(AGEs)作用下增殖活性的变化。方法:采用本实验室培养及鉴定的新生大鼠Mller细胞,设置正常生长组和添加AGEs培养组,AGEs浓度在250～1000mg/L范围内逐步递增。MTT法测定AGEs对体外纯化培养Mller细胞增殖的影响和分泌VEGF的改变。结果:高浓度AGEs对Mller细胞有明显的促增殖作用,并且促进Mller细胞分泌VEGF,并在一定的浓度范围内(250～1000mg/L)细胞增殖活性呈剂量依赖关系。结论:体外高浓度AGEs可促进Mller细胞异常增殖和VEGF表达增加,与临床上观察到增殖性糖尿病视网膜病变时胶质细胞反应性增生情况一致,因此可以在体外用高浓度AGEs模拟体内糖尿病环境,观察胶质细胞形态和功能的改变。
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| 关 键 词： | 糖基化终产物 Mller细胞 增殖 血管内皮细胞生长因子 |
Advanced glycosylation end products promote the growth of retinal Müller cells in vitro |
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| Hui Ye,Ge-Zhi Xu,Wen-Ji Wang.Advanced glycosylation end products promote the growth of retinal Müller cells in vitro[J].International Journal of Ophthalmology,2008,8(11):2217-2218. |
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| Authors: | Hui Ye Ge-Zhi Xu Wen-Ji Wang |
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| Institution: | 1Department of Ophthalmology,Jiangsu Provincial People’s Hospital,Nanjing 210029,Jiangsu Province,China;2Shanghai EENT Hospital Affiliated to Shanghai Fudan University,Shanghai 210023,China |
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| Abstract: | AIM:To observe the effect of advanced glycosylation end products (AGEs) on the growth of retinal glial cells in vitro.·METHODS:The Müller cells were exposed to different concentrations of AGEs (250-1000mg/L).And the normal Müller cells were as controls.The growth characteristics were measured with MTT assay and VEGF in medium concentration was assayed by ELISA.·RESULTS:It was demonstrated that AGEs with high concentration promoted the growth and VEGF of Müller cells and this effect was dosage dependent within 250-1000mg/L.·CONCLUSION:Interaction of Müller cells and AGEs is related to the formation and development of DR.· |
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| Keywords: | glycosylation end proclucts müller cells proliferation VEGF |
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