| 高效液相色谱法测定狼疮灵颗粒中芍药苷的含量 |
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| 引用本文： | 孙川,冯俭,李煌. 高效液相色谱法测定狼疮灵颗粒中芍药苷的含量[J]. 中国中医药信息杂志, 2011, 18(2): 66-67. DOI: 10.3969/j.issn.1005-5304.2011.02.027 |
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| 作者姓名： | 孙川 冯俭 李煌 |
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| 作者单位： | 1. 成都中医药大学药学院,四川,成都,610072
2. 成都中医药大学附属医院,四川,成都,610072 |
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| 摘 要： | 目的建立狼疮灵颗粒中芍药苷的含量测定方法。方法采用高效液相色谱法，色谱柱：Hypersil ODS-2（150mm×4.6mm，5．0um）；流动相：乙腈-0．4％的磷酸水溶液（10：90）；检测波长：230nm；流速：1．0mL／min，柱温：25℃。结果芍药苷在0．608～3．04ug范围内呈良好的线性关系（r=0．9999），加样回收率97．77％，RSD=0．62％（n=5）。结论该方法快速可行、简捷准确、重复性好、结果可靠，可用于控制狼疮灵颗粒的质量。
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| 关 键 词： | 狼疮灵颗粒 芍药苷 高效液相色谱法 含量测定 |
Determination of Paeoniflorin in Langchuangling Granules by HPLC |
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| SUN Chuan,FENG Jian,LI Huang. Determination of Paeoniflorin in Langchuangling Granules by HPLC[J]. Chinese Journal of Information on Traditional Chinese Medicine, 2011, 18(2): 66-67. DOI: 10.3969/j.issn.1005-5304.2011.02.027 |
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| Authors: | SUN Chuan FENG Jian LI Huang |
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| Affiliation: | SUN Chuan1,FENG Jian2,LI Huang1(1.Chengdu University of Traditional Chinese Medicine,Chengdu 610072,China,2.The Affiliated Hospital of Chengdu University of Traditional Chinese Medicine,China) |
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| Abstract: | Objective To establish an accurate method for determination of paeoniflorin in Langchuagrding Granules. Methods Hypersil ODS-2 （4.6 mm× 150 mm, 5.0 um ） in an oven at 25 ℃ was need, with a mobile phase consisting of acetonitril-0.4% granolite treatment （10 ： 90） and UV detector at 230 nm, the flow rate was 1.0 mL/min. Result The linear range of paeoniflorin was within 0.608-3.04 ug （r=0.9999）. The average recovery was 97.77% and RSD was 0.62% （n=5）. Conclusion The method is simple and accurate. It can be used for the quality control of Langchuagnling Granules. |
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| Keywords: | Langchuangling Granules paeoniflorin HPLC content determination |
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