| HPLC测定白芍配伍前后芍药苷的含量 |
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| 引用本文： | 王瑞,展晓日,刘真玮,曾昭武.HPLC测定白芍配伍前后芍药苷的含量[J].中国中医药信息杂志,2011,18(8):57-59. |
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| 作者姓名： | 王瑞 展晓日 刘真玮 曾昭武 |
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| 作者单位： | 1. 山西中医学院中药系,山西太原,030024
2. 杭州师范大学生物医药与健康中心,浙江杭州,310012 |
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| 基金项目： | 国家自然科学青年基金(81001647); 浙江省科技厅面上项目(2009C33005); 中国博士后科学基金面上项目(20100471757) |
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| 摘 要： | 目的比较白芍配伍前后芍药苷的含量变化,探讨小建中汤配伍的合理性。方法采用高效液相色谱法测定并比较小建中汤复方煎液和白芍单煎液中芍药苷的含量。采用Waters XTerra RP-C8色谱柱（3.9 mm×150 mm,5μm）,以乙腈-水为流动相,流速1.0 mL/min,检测波长232 nm。结果芍药苷在1.006～15.09μg范围内线性良好,白芍单煎液和复方煎液的回收率分别为97.2%和95.1%,单煎液中芍药苷的含量明显低于复方煎液芍药苷的含量（P〈0.01）。结论白芍在小建中汤中配伍可以增加有效成分芍药苷的煎出量。
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| 关 键 词： | 小建中汤 配伍 芍药苷 含量测定 高效液相色谱法 |
Different Contents of Paeoniflorin in Radix Paeoniae Alba before and after Compatibility Determined by HPLC |
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| WANG Rui,ZHAN Xiao-ri,LIU Zhen-wei,ZENG Zhao-wu.Different Contents of Paeoniflorin in Radix Paeoniae Alba before and after Compatibility Determined by HPLC[J].Chinese Journal of Information on Traditional Chinese Medicine,2011,18(8):57-59. |
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| Authors: | WANG Rui ZHAN Xiao-ri LIU Zhen-wei ZENG Zhao-wu |
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| Institution: | WANG Rui1,ZHAN Xiao-ri2,LIU Zhen-wei1,ZENG Zhao-wu2(1.Department of Chinese Materiac Media,Shanxi College of Traditional Chinese Medicine,Taiyuan 030024,China,2.Center of Biomedicine and Health,Hangzhou Normal University,Hangzhou 310012,China) |
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| Abstract: | Objective To compare the content of Paeoniflorin in Radix Paeoniae Alba and Xiaojianzhong decoction,which was composed of Radix Paeoniae Alba and other Chinese traditional medicines according to TCM theory,to explore the rationality of compatibility.Methods HPLC method was applied to determine the contents of Paeoniflorin in Xiaojianzhong decoction co-boiling extracts and Radix Paeoniae Alba individual boiling extracts.The difference of their contents of Paeoniflorin was compared.HPLC analysis was performed... |
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| Keywords: | Xiaojianzhong Decoction compatibility Paeoniflorin content determination HPLC |
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