麻黄碱壳聚糖修饰脂质体凝胶剂的制备及体外透皮研究

目的 制备麻黄碱壳聚糖修饰脂质体凝胶剂，考察其粒径分布、包封率及体外透皮特性。方法 采用薄膜蒸发法制备麻黄碱脂质体混悬液，在麻黄碱脂质体混悬液中缓慢滴加pH值为5～6的0.35%壳聚糖溶液，制成麻黄碱壳聚糖修饰脂质体凝胶剂。激光粒度仪测定修饰脂质体的粒径分布，反透析法测定包封率，Franz扩散装置考察其体外透皮特性。结果 麻黄碱壳聚糖修饰脂质体的平均粒径在1 400～1 900 nm；平均包封率达（41±0.94）%；修饰脂质体中麻黄碱能缓慢透过大鼠皮肤，与普通剂型相比累积透过量随时间变化差异较明显，缓释效果显著。结论 该制剂制备工艺简单，性质稳定，药物包封率较高，定量测定方法简便、准确；药物透皮速率缓慢，释药稳定。

Preparation and in vitro transdermal diffusion characteristics of ephedrine chitosan-modified liposomes gels

Objective To optimize the extraction technology of the active component, rosmarinic acid, an ester of caffeic acid and 3,4-dihydroxyphenyllactic acid, in perilla oil meal for the first time by a new homogenizing technology called smashing tissue extraction (STE). Methods Orthogonal design was used to optimize the extraction condition. The content of rosmarinic acid was quantified from the methanol crude extract with the help of HPLC. Results The optimization of STE process to get rosmarinic acid from the perilla oil meal was the ratio of liquid to solid material at 10:1 and the power of extraction at 150 V, extracting twice (2 min for each time). Conclusion STE could be applied to extracting the active ingredients from the oil meals due to its high extraction efficiency. This new homogenizing technology has advantages on saving extraction time, raising extraction efficiency, and maintaining the temperature sensitive constituents.