高效离子色谱-脉冲安培检测法测定食品中多聚葡萄糖

目的在AOAC2000.11的基础上,应用高效离子色谱-脉冲安培检器联用技术,建立食品中多聚葡萄糖的检测方法。方法匀质样品通过热水浸提、超滤离心、酶解等处理后,进样20μl,以0.15mol/LNaOH、含有0.15mol/LNaOH的0.5mol/L乙酸钠进行梯度淋漓,CarboPACTM PA1阴离子交换柱-金电极脉冲安培检测。结果方法检出限、定量限分别为1.69μg/g、5.47μg/g,重复性和复现性RSD2.10%~6.62%,不同食品基质多聚葡萄糖的平均加标回收率为92.4%~104.4%。结论该方法重现性良好,能够准确定量食品中多聚葡萄糖含量。

Determination of polydextrose in food by high performance anion exchange chromatographic method with pulsed amperometric detector

OBJECTIVE: The HPAEC-PAD method for polydextrose determination was developed based on AOAC 2000.11. METHODS: This method included water extraction, centrifugal ultrafiltration, mulienzyme hydrolysis, and anion exchange chromatography detection. The polydextrose was elated by a gradient program of 0.15 mol/L NaOH and 0.5mol/L sodium acetate in 0.15 mol/L NaOH on a CarboPAC TM PA 1 column, then detected by a gold electrode with pulse amperometric detection mold. The inject volume was 20 microl. RESULTS: The LOD and LOQ of this method were 1.69 microg/g, 5.47 microg/g, respectively. The repeatability and reproducibility were excellent, ranging from 2.10% to 6.62%. The average recovery of polydextrose in various food matrix were 92.4%-104.4%. CONCLUSION: This method could be used for polydextrose determination in foods.