| 骨形态发生蛋白-2基因转染对舌癌细胞增殖活性的影响 |
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| 引用本文： | Jiang J,Zhang J,Wang X,Xue L,Song H,Zhang X. 骨形态发生蛋白-2基因转染对舌癌细胞增殖活性的影响[J]. 华西口腔医学杂志, 2011, 29(5): 546-549. DOI: 10.3969/j.issn.1000-1182.2011.05.025 |
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| 作者姓名： | Jiang J Zhang J Wang X Xue L Song H Zhang X |
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| 作者单位： | 1.山东大学口腔医学院口腔颌面外科学教研所, 山东省口腔生物医学重点实验室, 济南250012;2.潍坊市人民医院口腔颌面外科, 潍坊261041 |
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| 基金项目： | 山东省科学技术发展计划基金资助项目(2010GSF10239) |
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| 摘 要： | 目的 探讨腺病毒介导的人骨形态发生蛋白-2 (BMP-2)基因转染舌癌Tca8113细胞后,对Tca8113细胞形态和增殖活性的影响.方法 分别以感染复数(MOI)为0、50、100的腺病毒为载体的BMP-2基因转染体外培养的舌癌Tca8113细胞.采用荧光倒置显微镜观察转染前后Tca8113细胞形态的变化,Weste...
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| 关 键 词： | 骨形态发生蛋白-2 腺病毒 基因转染 舌癌细胞 |
| 收稿时间： | 2011-10-25 |
| 修稿时间： | 2011-10-25 |
Effects of bone morphogenetic protein-2 on proliferation of tongue cancer Tca8113 |
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| Jiang Juan,Zhang Jun,Wang Xuxia,Xue Liwei,Song Hongning,Zhang Xi. Effects of bone morphogenetic protein-2 on proliferation of tongue cancer Tca8113[J]. West China journal of stomatology, 2011, 29(5): 546-549. DOI: 10.3969/j.issn.1000-1182.2011.05.025 |
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| Authors: | Jiang Juan Zhang Jun Wang Xuxia Xue Liwei Song Hongning Zhang Xi |
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| Affiliation: | 1. Dept. of Oral and Maxillofacial Surgery, School of Stomatology of Shandong University, Shandong Provincial Key Laboratory of Oral Biomedicine, Jinan 250012, China; 2. Dept. of Oral and Maxillofacial Surgery, People’s Hospital of Weifang, Weifang 261041, China） |
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| Abstract: | Objective To investigate the morphology and proliferation effects of adenovirus containing bone morpho-genetic protein-2(BMP-2) on tongue cancer Tca8113 cells.Methods Tca8113 cells were transfected with the AdBMP-2 of 0,50,100 multiplicity of infection(MOI) respectively.Inverted fluorescence microscope was used to evaluate the morphological changes of these cells.Western blot analysis was performed to determine the expression levels of BMP-2 in the transfected cells.Methyl thiazolyl tetrazolium(MTT) assay w... |
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| Keywords: | bone morphogenetic protein-2 adenovirus gene transfection tongue cancer cells |
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