癫痫患者血浆及红细胞中抗氧化剂水平的测定

目的：探讨癫痫疾病状态下血浆及红细胞（RBC）中抗氧化剂水平的变化。方法：测定39例正常对照组、32例癫痫疾病组和26例癫痫治疗组的RBC内谷胱甘肽还原酶(GR)、谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)、红细胞膜过氧化脂质代谢产物丙二醛（MDA）、红细胞溶血百分率以及血浆中铜蓝蛋白（CER）、维生素A,C,E(VitA、VitC、VitE)等。结果：疾病组的红细胞MDA,溶血百分率,GSH-Px,CAT及血浆CER显著高于对照组（P<0.05），红细胞GR、SOD和血浆VitA，VitC，VitE显著低于对照组（P<0.05,其中红细胞GR，血浆维生素C,P<0.01）；治疗组的红细胞GR,SOD，血浆VitC,VitE均显著高于疾病组(P<0.05,其中GR,P<0.01)。结论：提示采取抗氧化保护措施对患者的治疗有重要意义。

Determination of phenylalanine and tyrosine in serum by high-performance liquid chromatography with fluorescent detection

OBJECTIVE: To determine phenylalanine and tyrosine in serum by high-performance liquid chromatography (HPLC) method. METHODS: Serum precipitated with perchloric acid was isocratically eluted using a base-deactivated C8 column with 10% acetonitrile in water as the mobile phase. The flow rate was 1.0 ml/min. Fluorescent detector worked at an excitation wavelength of 210 nm and emission wavelength of 303 nm. RESULTS: Good linearity was observed in the range of 12.2 approximately 1 220.0 micromol/L for phenylalanine, the intra- and inter-assay coefficients of variation (CV) were 4.07% and 7.17% respectively, and the average recovery was 102.5%. There also was a good linearity in the range of 5.5 approximately 550.0 micromoL/L for tyrosine. The intra- and inter-assay coefficients of variation were 2.99% and 5.23% respectively, and the average recovery was 102.2%. The total assay time for the serum sample was within 30 min. We found a good correlation compared with HPLC ultraviolet detection. CONCLUSION: This method is simple, rapid, accurate in measuring phenylalanine and tyrosine in serum quantitatively.