Silk-elastinlike protein polymers for matrix-mediated cancer gene therapy |
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| Authors: | Joshua A. Gustafson Hamidreza Ghandehari |
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| Affiliation: | a Department of Bioengineering, University of Utah, Salt Lake City, UT, USAb Department of Pharmaceutics and Pharmaceutical Chemistry, University of Utah, Salt Lake City, UT, USAc Center for Nanomedicine, Nano Institute of Utah, University of Utah, Salt Lake City, UT, USA |
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| Abstract: | Silk-elastinlike protein polymers (SELPs) are recombinant polymers designed from silk fibroin and mammalian elastin amino acid repeats. These are versatile materials that have been examined as controlled release systems for intratumoral gene delivery. SELP hydrogels comprise monodisperse and tunable polymers that have the capability to control and localize the release and expression of plasmid DNA and viruses. This article reviews recent developments in the synthesis and characterization of SELP hydrogels and their use for matrix-mediated gene delivery. |
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| Keywords: | As(x), Aspartic acid or Asparagine 3S, 3 silk unit gene monomer A, Alanine Ad.HSVtk, Adenovirus carrying herpes simplex virus thymidine kinase gene Ad.GFP, Adenovirus carrying green fluorescent protein gene Ad.LacZ, Gene encoding for beta-galactosidase AFM, Atomic force microscopy AGR, Albumin/globulin ratio Ala, Alanine ALB, Albumin ALT, Alanine aminotransferase Arg, Arginine AST, Aspartate aminotransferase ATCC, American type culture collection BCA, Bicinchoninic acid bp, base pairs BUN, Blood urea nitrogen CAR, Coxsackie and adenovirus receptor CBC, Complete blood count CGC, Critical gelation concentration CRE, Creatinine D, Aspartic Acid Da, Dalton DMA, Dynamic mechanical analysis DNA, Deoxyribonucleic acid E, Glutamic Acid FDA, United States Food and Drug Administration G, Glycine GCV, Ganciclovir GDEPT, Gene-directed enzyme-prodrug therapy GFP, Green fluorescent protein Gl(x), Glutamic acid or Glutamine GLOB, Globulin Gly, Glycine GRA, Granulocyte H, Histidine HCT, Hematocrit His, Histidine HNSCC, Head and neck squamous cell carcinoma HSVtk, Herpes simplex virus thymidine kinase L, Leucine Leu, Leucine LYM, Lymphocyte Lys, Lysine M, Methionine MALDI-TOF, Matrix-assisted laser desorption-ionization-time-of-flight Met, Methionine MON, Mononuclear leukocyte N, Asparagine P, Proline PAMAM, Poly (amido amine) PBS, Phosphate buffered saline PCR, Polymerase chain reaction PFU, Plaque forming unit Phe, Phenylalanine PLT, Platelet pPT317, Expression vector for production of SELP pPT340, Plasmid encoding for SELP-415K Pro, Proline pSY1378, Plasmid encoding for six silk units Q, Glutamine q, Swelling ratio RBC, Red blood cell Res/Mol Ob, Observed amino acid residues per mole of protein Res/Mol Th, Theoretical amino acid residues per mole of protein RT-PCR, Real-time PCR S, Serine SANS, Small-angle neutron scattering SAP, Shrimp Alkaline Phosphatase SDS-PAGE, Sodium dodecyl sulfate-polyacrylamide gel electrophoresis SELP, Silk-elastinlike protein polymer Ser, Serine SLP6, Six silk unit monomer T, Threonine TBIL, Total bilirubin Thr, Threonine TP, Total protein Trp, Tryptophan Tyr, Tyrosine V, Valine Val, Valine W, Tryptophan WBC, White blood cell Y, Tyrosine β-gal, Beta-galactosidase |
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