Localisation of COX-2 protein is different in breast ductal carcinoma and adjacent non-tumour ductal epithelium |
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Authors: | P. Cejas M. A. García-Cabezas E. Casado J. Barriuso J. A. Fresno E. Díaz C. Belda-Iniesta J. Castro E. Espinosa P. Zamora J. Feliu A. Redondo D. A. Hardisson M. González-Barón |
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Affiliation: | (1) Servicio de Oncología Médica. Unidad de Oncología Molecular. Hospital Universitario La Paz, Universidad Autónoma de Madrid, C/ Paseo de la Castellana, 261, Madrid, Spain;(2) Departmento de Patología, Hospital Universitario La Paz, Madrid, Spain |
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Abstract: | Introduction A number of findings suggest that cyclooxygenase-2 (COX-2) is overexpressed in breast tumours. However, there is a lack of consensus in the literature regarding the pattern of expression of this protein in invasive breast ductal carcinoma and in the adjacent non-tumour ductal epithelium. This study compares the expression of COX-2 mRNA and protein in breast ductal carcinoma relative to non-tumour breast tissue. Material and methods We analysed the expression of COX-2 mRNA by quantitative PCR, and COX-2 protein by immunohistochemistry in invasive ductal carcinoma as well as in non-tumour adjacent ductal epithelium from 54 breast biopsies diagnosed as being invasive ductal carcinoma. As control, we analysed expression of COX-2 protein by immunohistochemistry in surgically-resected benign breast lesions. Results Our results show that COX-2 mRNA and protein are overexpressed in non-tumour ductal epithelium compared with invasive ductal carcinoma. However, the pattern of the protein expression is different in tumour and non-tumour tissue: COX-2 protein is expressed predominantly in the membrane of the non-tumour ductal epithelium (including in benign breast lesions) while, in invasive ductal carcinoma cells, it is localised in the cytoplasm. Conclusions The non-tumour ductal epithelium adjacent to invasive ductal carcinoma shows a higher COX-2 expression than does the invasive ductal carcinoma. However, the different localisation of the immunohistochemically-detected protein suggests a possible post-translational regulation of the protein. |
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Keywords: | breast cancer COX-2 carcinogenesis, ductal carcinoma |
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