碘化钾对人体皮肤成纤维细胞增殖活性的影响

目的探讨不同浓度的碘化钾（KI）对人体皮肤成纤维细胞(HSF)增殖活性的影响。方法将体外培养的ＨＳＦ分成7组含不同浓度的KI（0、1?000、3?000、5?000、7?000、9?000、11?000?μg/L），每组含8个平行样本。完全培养基分别培养24?h后，观察HSF形态变化，用流式细胞术和酶联免疫吸附试验（ELISA）方法检测HSF增殖活性。结果KI的浓度在1?000、3?000、5?000?μg/L时成纤维的增殖活性随着碘浓度的增加而升高（P＜0.05）；5?000、7?000?μg/L组细胞增殖活性最高，两组间增殖活性差异无统计学意义（P＞0.05）；7?000、9?000、11?000?μg/L?时HSF增殖活性随着KI浓度的增加而降低（P＜0.05）。结论KI浓度在5?000?μg/L以下会促进HSF增殖活性，高于7?000?μg/L时反而会抑制HSF增殖活性。

Effect of KI on proliferative activity of human skin fibroblasts

To study the effects of KI on proliferative activity of human skin fibroblasts at different doses. MethodsHuman skin fibroblasts (HSF) were treated with KI 0, 1?000,3?000,5?000,7?000,9?000, and 11?000?μg/L for 24?h and to observe the morphology of fibroblasts. The methods of FCM and ELISA were used to detect the proliferation activity of human skin fibroblasts. ResultsKI at a certain range of concentration(＜5?000?μg/L)could significantly increase proliferative activity of human skin fibroblasts but decreases the proliferative activity at a certain range of concentration(＞7?000?μg/L). The proliferative activity was the highest from 5?000?μg/L to 7?000?μg/L, but there were no significant differences between them. ConclusionKI with an exposure level＜5?000?μg/L can increase the proliferative activity of human skin fibroblasts, whereas it decrease the activity when its exposure ＞7?000?μg/L.