双氢睾酮对前列腺癌LNCaP细胞线粒体呼吸链酶学变化的影响

目的 观察双氢睾酮对人前列腺癌细胞LNCaP细胞株线粒体呼吸链酶学变化的影响,探讨双氢睾酮引起LNCaP线粒体内酶学变化的作用机制.方法 获得雄激素依赖性细胞(LNCaP)细胞株,进行细胞培养,获得稳定生长及传代的细胞株.不同浓度的雄激素受体激动剂双氢睾酮作用于培养的LNCaP细胞株;不同时间点提取干预措施下的LNCaP线粒体;检测并比较不同时间点、不同浓度药物作用下LNCaP线粒体呼吸链酶学的变化和差异.结果 DHT作用于LNCaP细胞后,线粒体蛋白的含量显著增加(P＜0.05),但SDH、NADH、COX的含量显著下降(P＜0.05),各个指标在不同的时间点上下降的程度有所差异.结论 双氢睾酮可引起LNCaP线粒体内蛋白的表达量升高,促进LNCaP的增殖.

Effects of DHT on the mitochondrial respiratory chain enzyme in prostate cancer cells LNCaP

Objectives To explore the effects of DHT on the mitochondrial respiratory chain enzyme in prostate cancer cells LNCaP,and to investigate its mechanisms.Methods Drogen-dependent(LNCaP) prostate cancer cell lines was obtained by cell culture.The androgen receptor agonist DHT roles in the culture prostate cancer cell line of androgen at different concentrations drugs and mitochondria at different time points of LNCaP under the interventions were extracted,and mitochondrial respiratory changes and differences at different time points and under the action of different drug concentration in LNCaP were measured and compared.Results After DHT acts on LNCaP cells,the content of mitochondrial protein increased significantly(P ＜ 0.05),but the content of SDH、NADH、COX decreased significantly(P ＜ 0.05).The difference of the decreasing degree of each index in different time points has different.Conclusions DHT can increase expression of mitochondrial protein in LNCaP,and promote cell proliferation.