免疫透射比浊法多点非线性校准在脂蛋白(a)测定中的应用 |
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引用本文: | 张汉园,曹风华,王斌. 免疫透射比浊法多点非线性校准在脂蛋白(a)测定中的应用[J]. 国际检验医学杂志, 2016, 0(22). DOI: 10.3969/j.issn.1673-4130.2016.22.016 |
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作者姓名: | 张汉园 曹风华 王斌 |
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作者单位: | 江苏省镇江市中西医结合医院检验科 212003 |
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摘 要: | 目的探讨免疫透射比浊法测定脂蛋白(a)中,采用2种多点非线性校准方法的测定结果,并确定最合适的校准曲线。方法分别采用Y=aX~2+bX+c和Spline2种方法在AU680生化仪上进行脂蛋白(a)校准。分别检测200例来自临床患者的血清标本和4个标准定值血清,200例临床患者血清按照测定值高低分为5个不同梯度水平,分别统计2组数据的均值(x)、标准差(s),相关系数(r),相对误差(v),偏倚(%)。采用SPSS19.0软件对2组数据偏倚进行统计学分析,以P0.05表示差异有统计学意义。结果 200例临床患者数据表明,采用Y=aX~2+bX+c曲线时,0~100、400~600、600~800mg/L组的脂蛋白(a)测定值比Spline曲线所得数值高;100~200mg/L组和200~400mg/L组的脂蛋白(a)测定值相反,Spline曲线所得数值较高。4个标准定值血清的测定结果,Spline曲线所得测定值更接近靶值;而Y=aX~2+bX+c曲线所测定结果中,100~200mg/L组和300~400mg/L组所得值偏低,600~800mg/L组所得值偏高,这与临床患者的血清标本结果一致。结论 Spline曲线更为客观,能更准确地体现免疫透射比浊法测定脂蛋白(a)整个反应过程。
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关 键 词: | 非线性校准 脂蛋白(a) 免疫透射比浊法 |
Application of immune transmission turbidimetry multipoint nonlinear calibration in lipoprotein (a) determination |
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Abstract: | Objective To adopt the results detected by the two kinds of multipoint nonlinear calibration method in immune transmission turbidimetry for detecting lipoprotein(a) and to ascertain the most suitable calibration curve .Methods The two kinds of method Y=aX2 + bX+ c and Spline were adopted to conduct the lipoprotein (a) calibration in the AU680 biochemical instru‐ment .Two hundreds serum samples from clinical patients and 4 standard fixed value serum specimens were detected respectively . The sera from 200 cases of clinical patients were divided into 5 different gradient levels .The mean value(x) ,standard deviation(s) , correlation coefficient(r) ,relative error(v) ,bias(% ) were respectively performed statistics .The biases in the two groups of data were statistically analyzed by using the SPSS19 .0 software ,with P<0 .05 expressing that the difference having statistical signifi‐cance .Results The data in 200 clinical patients indicated that using the curve of Y=aX2 +bX+c ,the lipoprotein(a) detection val‐ues in the 0-100 mg/L group ,400-600 mg/L group and 600-800 mg/L group were higher than those by the Spline curve ;while the detection values in the 100-200 mg/L group and 200-400 mg/L group were on the contrary ,the values obtained by using the Spline curve were higher .In the detection results of 4 standard fixed value sera ,the values detected by the Spline curve were more close to the target values ;while in the results detected by using the curve of Y=aX2 +bX+c ,the values in the 100 mg/L group and 300 mg/L group were on the low side and the values in the 600 mg/L group were on the high side ,which were consistent with the serum specimen results from clinical patients .Conclusion The Spline curve is more objective and can accurately embody the whole reaction process of lipoprotein (a) detection by the immune transmission turbidimetry . |
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Keywords: | nonlinear calibration lipoprotein(a) immune transmission turbidimetry |
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