2种检测胃蛋白酶原方法的比较 |
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引用本文: | 张莲英,肖虎,鲁旭,肖林林. 2种检测胃蛋白酶原方法的比较[J]. 国际检验医学杂志, 2016, 0(20): 2810-2812. DOI: 10.3969/j.issn.1673-4130.2016.20.003 |
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作者姓名: | 张莲英 肖虎 鲁旭 肖林林 |
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作者单位: | 1. 上海交通大学附属第六人民医院南院 输血科 201499;2. 上海交通大学附属第六人民医院南院 检验科 201499 |
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摘 要: | 目的探讨胶体金免疫层析法和酶联免疫吸附法(ELISA)检测胃蛋白酶原(PG)结果的一致性,为胶体金免疫层析法检测PG的可靠性提供实验依据。方法选取该院消化内镜中心经胃镜确诊的胃部炎性患者40例和体检中心体检健康者60例,分别采用胶体金免疫层析法和ELISA法检测2组研究对象的PG,胶体金免疫层析法为待评价方法,ELISA法为参考方法,对2组结果进行方法比对和偏倚分析。结果 2种方法检测结果的相关系数均大于0.95,说明相关性良好且不存在整体偏倚;2种方法的敏感性和特异性分别为0.775、0.85和0.782、0.823。结论 2种方法均可作为胃病患者的辅助诊断方法。
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关 键 词: | 胃蛋白酶原 胶体金免疫层析法 酶联免疫吸附法 |
The comparison of two methods of detecting pepsinogen |
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Abstract: | Objective To investigate colloidal gold immune chromatography (GICA ) and enzyme linked immunosorbent assay (ELISA) detection pepsinogen (PG) consistency of results ,so as to provide experimental evidence for the use of colloidal gold im‐munochromatography assay PG and briefly analysis of its clinical utility .Methods Totally 40 cases in our hospital digested samples and medical centers 60 healthy population sample in Endoscopy Center by endoscopy diagnosed with inflammation of the stomach disease ,respectively ,with colloidal gold immune chromatography and enzyme‐linked immunosorbent assay PG ,colloidal gold immu‐noassay chromatography method to be evaluated ,as the reference ELISA method .The results were compared to the method and bias analysis .Results GICA method with a correlation coefficient ELISA test results were greater than 0 .95 ,indicating a good correla‐tion between the two methods and there is no overall bias;sensitivity and specificity of the two methods were (0 .775 ,0 .85) and (0 .782 ,0 .823) .Conclusion Both GICA and ELISA can be used for screening or diagnosis of stomach illnesses . |
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Keywords: | pepsinogen GICA methods ELISA |
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