| 乙型肝炎病毒B、C基因型全基因组序列的克隆 |
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| 引用本文： | 赵建华,陆仁飞. 乙型肝炎病毒B、C基因型全基因组序列的克隆[J]. 国际检验医学杂志, 2016, 0(22): 3101-3104. DOI: 10.3969/j.issn.1673-4130.2016.22.004 |
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| 作者姓名： | 赵建华 陆仁飞 |
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| 作者单位： | 江苏省南通市第三人民医院检验科 226006 |
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| 基金项目： | 江苏省南通市卫生和计划生育委员会资助项目(WQ2014038，W201217)；江苏省南通市科技局资助项目(HS2014062)。 |
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| 摘 要： | 目的构建乙型肝炎病毒(HBV)B、C基因型全基因组序列克隆。方法从HBV无症状慢性携带者中筛选B、C基因型,提取病毒核酸,设计引物,应用高保真酶对3 200bp的HBV DNA进行全序列扩增,通过克隆技术构建pGEM-HBV重组质粒,测序后进行序列分析。结果获得HBV B基因型和HBV C基因型重组质粒各1株。结论成功构建HBV B基因型和HBV C基因型的全基因组序列克隆,可为进一步研究HBV分子流行病学和基础研究提供工具。
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| 关 键 词： | 乙型肝炎病毒 B基因型 C基因型 全基因组序列 克隆 |
Cloning of complete-genome sequence of hepatitis B virus genotype B and C |
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| Abstract: | Objective To construct the clone of full‐genome sequence of hepatitis B virus (HBV ) genotype B and C . Methods HBV genotype B and C were screened from asymptomatic carriers of HBV for extracting HBV nucleic acid and designing primer .The high fidelity enzyme was used to perform the full sequence amplification of 3 200 bp HBV DNA .The pGEM‐HBV re‐combinant plasmid was constructed by using the clone technique .Then the sequence analysis was performed after sequencing .Results Each strain of recombinant HBV genotype B and C was obtained .Conclusion The clone of full‐genome sequence of HBV genotype B and C is successfully constructed ,which can provide a tool for further study of HBV molecular epidemiology and basic study . |
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| Keywords: | HBV genotype B genotype C complete genome sequence clone |
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